LOCUS       Exported                9545 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  PiggyBac cloning vector pHomeT-1-R, complete sequence.
ACCESSION   HQ159398
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9545)
  AUTHORS   Windbichler N, Menichelli M, Papathanos PA, Thyme SB, Li H, Ulge UY,
            Hovde BT, Baker D, Monnat RJ Jr., Burt A, Crisanti A.
  TITLE     A synthetic homing endonuclease-based gene drive system in the human
            malaria mosquito
  JOURNAL   Nature 473 (7346), 212-215 (2011)
  PUBMED    21508956
REFERENCE   2  (bases 1 to 9545)
  AUTHORS   Windbichler N.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2010) Imperial College London, Imperial College 
            Road, London SW7 2AZ, England
REFERENCE   3  (bases 1 to 9545)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9545
                     /organism="piggyBac cloning vector pHomeT-1-R"
                     /mol_type="other DNA"
                     /db_xref="taxon:935899"
     CDS             20..739
                     /codon_start=1
                     /product="GFP"
                     /label=GFP
                     /protein_id="ADT78591.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    862..983
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      1023..3632
                     /regulatory_class="promoter"
                     /gene="actin 5C"
     gene            1023..3632
                     /gene="actin 5C"
                     /label=actin 5C
     promoter        1051..3628
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             3640..4320
                     /codon_start=1
                     /product="RFP"
                     /label=RFP
                     /protein_id="ADT78590.1"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     polyA_signal    4488..4918
                     /label=hsp70 poly(A)
                     /note="polyadenlyation signal from the Drosophila 
                     melanogaster hsp70Ab gene"
     protein_bind    6038..6107
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(6143..6159)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6167..6183
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6191..6221)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6236..6257
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6545..7133)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7304..8164)
                     /codon_start=1
                     /gene="ampR"
                     /product="AmpR"
                     /label=ampR
                     /protein_id="ADT78589.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(7304..8164)
                     /gene="ampR"
                     /label=ampR
     promoter        complement(8165..8269)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      9245..9505
                     /regulatory_class="promoter"
                     /gene="3xP3"
     gene            9245..9505
                     /gene="3xP3"
                     /label=3xP3