LOCUS       Exported                3523 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHK, complete sequence.
ACCESSION   KY792637
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Zhang J, Liu S, Li R, Hong W, Xiao Y, Feng Y, Cui Q, Liu YJ.
  TITLE     Efficient whole-cell-catalyzing cellulose saccharification using 
            engineered Clostridium thermocellum
  JOURNAL   Biotechnol Biofuels 10, 124 (2017)
  PUBMED    28507596
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Zhang J, Liu S, Li R, Hong W, Xiao Y, Feng Y, Cui Q, Liu Y-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2017) Metabolomics Group, Qingdao Institute of 
            Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 
            189 Songling Rd., Qingdao 266101, P. R. China
REFERENCE   3  (bases 1 to 3523)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Cloning vector pHK"
                     /mol_type="other DNA"
                     /db_xref="taxon:2005743"
     protein_bind    110..131
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(261..911)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /protein_id="ARU79090.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     gene            complement(261..911)
                     /gene="cat"
                     /label=cat
     CDS             complement(1018..2022)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein"
                     /label=repB
                     /protein_id="ARU79089.1"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     gene            complement(1018..2022)
                     /gene="repB"
                     /label=repB
     rep_origin      complement(2714..3302)
                     /direction=LEFT