LOCUS       Exported                3073 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHIT88 DNA, complete sequence.
ACCESSION   AB372858
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Aoki K, Moriguchi H, Yoshioka T, Okawa K, Tabara H.
  TITLE     In vitro analyses of the production and activity of secondary small 
            interfering RNAs in C. elegans
  JOURNAL   EMBO J. 26 (24), 5007-5019 (2007)
  PUBMED    18007599
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Tabara H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2007) Contact:Hiroaki Tabara Kyoto University, 
            Graduate School of Medicine; Yoshida-Konoe, Building E, Room 208, 
            Sakyo, Kyoto 606-8501, Japan Fax :81-75-753-9311
REFERENCE   3  (bases 1 to 3073)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Cloning vector pHIT88"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /note="pHIT88 is a plasmid vector designed for in vitro 
                     synthesis of mRNA from nematode C. elegans. This plasmid is
                     derived from pCITE-4b."
                     /db_xref="taxon:490052"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1..18
                     /regulatory_class="promoter"
                     /note="T7 RNA polymerase promoter"
     misc_feature    18..39
                     /label=trans-splicing leader sequence SL1
                     /note="trans-splicing leader sequence SL1"
     misc_feature    38..59
                     /note="multiple cloning sites containing restriction sites 
                     (Stu I, Pst I, Xho I and Xba I)"
     misc_feature    59..119
                     /label=synthetic poly(A)61 region
                     /note="synthetic poly(A)61 region"
     misc_feature    115..131
                     /note="restriction enzyme sites (Bsa I and Spe I) for 
                     linearization of the plasmid"
     regulatory      146..193
                     /regulatory_class="terminator"
                     /note="T7 RNA polymerase terminator"
     terminator      146..193
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(393..981)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1152..2012)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="ampicillin resistance gene"
                     /protein_id="BAF95892.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2013..2117)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2451..2906)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     3051..3067
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"