LOCUS       Exported                4613 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHIS3NB, complete sequence.
ACCESSION   AF272029
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Meijer AH, Ouwerkerk PB, Hoge JH.
  TITLE     Vectors for transcription factor cloning and target site 
            identification by means of genetic selection in yeast
  JOURNAL   Yeast 14 (15), 1407-1415 (1998)
  PUBMED    9848232
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Ouwerkerk PB.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2000) Molecular Cell Biology, Institute of 
            Molecular Plant Sciences, Wassenaarseweg 64, Leiden 2333 AL, 
            Netherlands
REFERENCE   3  (bases 1 to 4613)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Cloning vector pHIS3NB"
                     /mol_type="genomic DNA"
                     /note="pHIS3NB is one of the components of an integration 
                     vector system for one-hybrid screenings in yeast and can be
                     used in combination with the integration vector pINT1 
                     deposited in GenBank Accession Number AF289993; an 
                     alternative for pHIS3NB is pHIS3NX deposited in GenBank 
                     Accession Number AF272030"
                     /db_xref="taxon:133221"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      complement(950..1589)
                     /regulatory_class="terminator"
     CDS             complement(1590..2249)
                     /codon_start=1
                     /gene="HIS3"
                     /product="imidazole glycerol-phosphate dehydratase"
                     /label=HIS3
                     /protein_id="AAF97426.1"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
                     ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     gene            complement(1590..2249)
                     /gene="HIS3"
                     /label=HIS3
     misc_feature    complement(2349..2396)
                     /note="multiple cloning site; unique sites are NotI, XbaI, 
                     SpeI, SmaI, EcoRI"
     primer_bind     complement(2372..2388)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2425..2443)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2464..2480)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2488..2504
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2512..2542)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2557..2578
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2866..3454)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3625..4485)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4486..4590)
                     /gene="bla"
                     /label=AmpR promoter