LOCUS       Exported                3656 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHIND2.2, complete sequence.
ACCESSION   AF128862
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   McNulty JJ, Dunn JJ, Randesi M.
  TITLE     Development of the pHIND series of plasmids useful for generating 
            nested deletions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   McNulty JJ, Dunn JJ, Randesi M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-1999) Biology, Brookhaven National Laboratory, 50 
            Bell Ave., Upton, NY 11973, USA
REFERENCE   3  (bases 1 to 3656)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Cloning vector pHIND2.2"
                     /mol_type="genomic DNA"
                     /label=allows nested deletions to be performed
                     /note="allows nested deletions to be performed"
                     /db_xref="taxon:92756"
     protein_bind    68..89
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        104..134
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    142..158
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     166..182
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             178..489
                     /codon_start=1
                     /gene="lacZ"
                     /product="LacZ"
                     /label=lacZ
                     /note="LacZ alpha complement region; used for color 
                     selection of insert-bearing clones"
                     /protein_id="AAD31805.1"
                     /translation="MTMITPSYLGDTIEYSSLHACRSTLEDPRVPSSNSPYSESYYNSL
                     AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWTRPVAAH"
     gene            178..489
                     /gene="lacZ"
                     /label=lacZ
     regulatory      200..222
                     /regulatory_class="promoter"
                     /note="SP6 promoter"
     promoter        200..218
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    225..281
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     regulatory      complement(280..302)
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     promoter        complement(284..302)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(309..325)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(619..1434)
                     /codon_start=1
                     /gene="kanR"
                     /product="kanamycin resistance protein"
                     /label=kanR
                     /protein_id="AAD31806.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(619..1434)
                     /gene="kanR"
                     /label=kanR
     rep_origin      1574..2449
                     /label=ColE1
                     /note="ColE1"
     rep_origin      complement(1736..2324)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2495..3355)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="confers ampicillin resistance"
                     /label=bla
                     /protein_id="AAD31807.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRILYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2495..3355)
                     /gene="bla"
                     /label=bla
     promoter        complement(3356..3460)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3557..3573
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3592..3642
                     /note="F1 phage; used for specifically nicking DNA"