LOCUS       Exported               11635 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHelper, complete sequence.
ACCESSION   AF369965
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11635)
  AUTHORS   Grafsky AJ III.
  TITLE     pHelper for use in the AAV Helper-Free System
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11635)
  AUTHORS   Grafsky AJ III.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2001) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   3  (bases 1 to 11635)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11635
                     /organism="Cloning vector pHelper"
                     /lab_host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:161366"
     misc_feature    complement(1..5336)
                     /label=similar to Adenovirus-5 E2A
                     /note="similar to Adenovirus-5 E2A"
     misc_feature    complement(5336..8537)
                     /label=similar to Adenovirus-5 E4
                     /note="similar to Adenovirus-5 E4"
     misc_feature    complement(8537..9280)
                     /label=similar to Adenovirus-5 VA
                     /note="similar to Adenovirus-5 VA"
     rep_origin      complement(9315..10167)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="ColE1 origin"
     rep_origin      complement(9423..10011)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10182..11042)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="confers ampicillin resistance"
                     /protein_id="AAK76423.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11043..11147)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11172..11627
                     /label=f1 origin
                     /note="f1 origin"
     rep_origin      complement(11173..11627)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"