LOCUS       Exported                5510 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pHel3, complete sequence.
ACCESSION   MG214727
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Haas R, Heuermann D.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2017) Genetics and Genomics, Icahn School of 
            Medicine at Mount Sinai, 1425 Madison Ave, New York, NY 10029, USA
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: HGAP v. 3
            Coverage              :: 100x
            Sequencing Technology :: PacBio
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Shuttle vector pHel3"
                     /lab_host="Escherichia coli DH5a"
                     /mol_type="other DNA"
                     /db_xref="taxon:2056838"
     rep_origin      complement(266..885)
                     /direction=LEFT
                     /label=pBR322_origin
                     /note="pBR322_origin"
     rep_origin      complement(281..869)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      complement(1059..1157)
                     /direction=LEFT
                     /label=oriT_RP4
                     /note="oriT_RP4"
     oriT            1095..1204
                     /note="incP origin of transfer"
     CDS             2082..3701
                     /codon_start=1
                     /product="RepA"
                     /label=RepA
                     /protein_id="ATY70547.1"
                     /translation="MPMNTNFEQLRKQELELRKLLEELETLPQTPQIKLQKQKIQTYID
                     KITPSILSGFDQKFKEIIENLSNEFEKEKSTPLKEPQTTPTPCKDLVVSTPKDNTYTTY
                     HNNANKVNLGKLSEREANLLFAIFQKLKDQGNTLIRFEPQDLKRMIMVKSNLTNRQLLQ
                     ILKNLLDNIGGANFWIIREHVENGEIYEDHTSYMLFKQFDIRIHKPTQTIEYLEVQLND
                     SYHYLLNNLGMGGQYTSFKLLEFQRVRGKYAKTLYRLLKQYKSTGILSVEWDQFRELLD
                     IPKDYKMENIDQKVLTPSLKELRKIYPFEHLSYKKERKSHDKRKVTHIDFYFEQLPQGE
                     TKKQKQADKQRAQRDIKLIAWDIHNQIAKRNAKATIEARFLELKTLIGYQFRHNNGTIL
                     QIDNATFEKNQMFLHVSTSKNSQKFLVSNKTFALELLFVNGYSLKKDMLEIDPPSIHPI
                     TNEPIKEFAEYIGKTIHITNFNVDQCPEGINNYLKITRIVKLNDNRICVSVQDVDKPEK
                     LLKPFIAKDEKHLKNWFKKHYR"
     CDS             complement(4227..5021)
                     /codon_start=1
                     /product="AphA-3"
                     /label=AphA-3
                     /protein_id="ATY70548.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"