LOCUS       Exported                6838 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  GFP expression vector pHapII, complete sequence.
ACCESSION   HM151400
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6838)
  AUTHORS   Zhang N, Wu K, He X, Li S-Q., Zhang Z-H., Shen B, Yang Y-M., Zhang 
            R-F., Huang Q-W., Shen Q-R.
  TITLE     A new bioorganic fertilizer can effectively control banana wilt by 
            strong colonization with Bacillus subtilis N11
  JOURNAL   Plant Soil 344 (1-2), 87-97 (2011)
REFERENCE   2  (bases 1 to 6838)
  AUTHORS   Zhang Z, Tian W, Liu D.
  TITLE     Development of a GFP vector system in Bacillus under the control of 
            the constitutive promoter HpaII
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6838)
  AUTHORS   Zhang Z, Tian W, Liu D, Zhou T, Wu Y, Liu Y, Xu L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2010) Jiang Su Provincial Key Lab for Organic 
            Solid Waste Utilization, Nanjing Agricultural University, Wei Gang 
            #1, Nan jing, Jiang Su 210095, China
REFERENCE   4  (bases 1 to 6838)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6838
                     /organism="GFP expression vector pHapII"
                     /lab_host="Bacillus spp.; Escherichia coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:866769"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             814..1818
                     /codon_start=1
                     /gene="repB"
                     /product="RepB replication protein"
                     /label=repB
                     /note="from Enterococcus faecalis plasmid pAM-alpha-1"
                     /db_xref="GI:22652809"
                     /protein_id="AAN03827.1"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     CDS             1111..1818
                     /codon_start=1
                     /product="repB"
                     /label=repB
                     /protein_id="ADK73493.1"
                     /translation="MKHGIQSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSD
                     MAQGFRRMMQYKKINKNLVGFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVN
                     QKQWIQFWKKAMKLDYDPNVKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEK
                     NLKRLSDLEEGLHRKRLISYGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIA
                     MWNWERKNYFIKE"
     CDS             1987..2748
                     /codon_start=1
                     /product="kanamycin nucleotidyltranferase"
                     /label=kanamycin nucleotidyltranferase
                     /note="KM"
                     /protein_id="ADK73494.1"
                     /translation="MNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGRQTD
                     GPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFDSEEILLDYASQVESDWPLTHGQFF
                     SILPIYDSGGYLEKVYQTAKSVEAQTFHDAICALIVEELFEYAGKWRNIRVQGPTTFLP
                     SLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQSDLPSGYDHLCQFVMSGQLSDSEK
                     LLESLENFWNGIQEWTERHGYIVDVSKRIPF"
     CDS             2965..3216
                     /codon_start=1
                     /product="ble"
                     /label=ble
                     /protein_id="ADK73495.1"
                     /translation="MRMLQSIPALPVGDIKKSIGFYWDKVGFTLVHHEDGFAVLMCNEV
                     RIHLWEASDEGWRLVVMIHRFVQVRSRLLLVLLVAALK"
     CDS             3873..4589
                     /codon_start=1
                     /product="GFP"
                     /label=GFP
                     /protein_id="ADK73496.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4617..4633)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4641..4657
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4665..4695)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4710..4731
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5019..5607)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5778..6638)
                     /codon_start=1
                     /gene="bla"
                     /product="bla"
                     /label=bla
                     /protein_id="ADK73497.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(5778..6638)
                     /gene="bla"
                     /label=bla
     promoter        complement(6639..6743)
                     /gene="bla"
                     /label=AmpR promoter