LOCUS       Exported                3319 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pHA-SP, complete sequence.
ACCESSION   AF531171
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Reverte CG, Yuan L, Keady BT, Lacza C, Attfield KR, Mahon GM, 
            Freeman B, Whitehead IP, Hake LE.
  TITLE     XGef is a CPEB-interacting protein involved in Xenopus oocyte 
            maturation
  JOURNAL   Dev. Biol. 255 (2), 383-398 (2003)
  PUBMED    12648498
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Reverte CG, Hake LE.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2002) Biology, Boston College, 140 Commonwealth 
            Avenue, Chestnut, MA 02467, USA
REFERENCE   3  (bases 1 to 3319)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Expression vector pHA-SP"
                     /lab_host="Xenopus laevis"
                     /mol_type="genomic DNA"
                     /note="modified version of pSP64-S that is related to 
                     pSP64T"
                     /db_xref="taxon:211917"
     regulatory      1..20
                     /regulatory_class="promoter"
                     /note="SP6"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     5UTR           26..73
                     /label=globin
                     /note="globin"
     5UTR           31..73
                     /label=Xenopus globin 5-UTR
                     /note="translational enhancer from the 5-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     misc_feature    93..125
                     /note="HA-tag; encodes hemaglutanin tag for 
                     immunodetection"
     CDS             99..125
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    126..159
                     /label=multiple cloning site
                     /note="multiple cloning site"
     3UTR           171..301
                     /label=globin
                     /note="globin"
     3UTR           171..296
                     /label=Xenopus globin 3-UTR
                     /note="3-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     misc_feature    302..334
                     /label=poly A tail
                     /note="poly A tail"
     misc_feature    352..387
                     /note="contains multiple RNA linearization sites for in 
                     vitro transcription"
     primer_bind     complement(406..422)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    430..446
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(454..484)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    499..520
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(808..1396)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1567..2427)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="confers ampicilin resistance"
                     /label=bla
                     /protein_id="AAO83380.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(1567..2427)
                     /gene="bla"
                     /label=bla
     promoter        complement(2428..2532)
                     /gene="bla"
                     /label=AmpR promoter