LOCUS       Exported                5834 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  HIS3/URA3 reporter vector pH3U3, complete sequence.
ACCESSION   DQ515893
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5834)
  AUTHORS   Meng X, Brodsky MH, Wolfe SA.
  TITLE     A bacterial one-hybrid system for determining the DNA-binding 
            specificity of transcription factors
  JOURNAL   Nat. Biotechnol. 23 (8), 988-994 (2005)
  PUBMED    16041365
REFERENCE   2  (bases 1 to 5834)
  AUTHORS   Meng X, Brodsky MH, Wolfe SA.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2006) Program in Gene Function and Expression, 
            University of Massachusetts Medical School, 364 Plantation St., 
            Worcester, MA 01605, USA
REFERENCE   3  (bases 1 to 5834)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5834
                     /organism="HIS3/URA3 reporter vector pH3U3"
                     /mol_type="other DNA"
                     /db_xref="taxon:382946"
     terminator      12..98
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(633..1583)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      1631..1853
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      2345..2439
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2470..3264)
                     /codon_start=1
                     /gene="kan"
                     /product="kanamycin resistance protein"
                     /label=kan
                     /protein_id="ABF48725.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            complement(2470..3264)
                     /gene="kan"
                     /label=kan
     terminator      3419..3505
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3597..3624
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3785..3815
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     3844..3860
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3856..4518
                     /codon_start=1
                     /gene="HIS3"
                     /product="HIS3"
                     /label=HIS3
                     /protein_id="ABF48726.1"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     gene            3856..4518
                     /gene="HIS3"
                     /label=HIS3
     CDS             4566..5369
                     /codon_start=1
                     /gene="URA3"
                     /product="URA3"
                     /label=URA3
                     /protein_id="ABF48727.1"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLATGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     gene            4566..5369
                     /gene="URA3"
                     /label=URA3
     rep_origin      complement(5378..5833)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"