LOCUS       Exported               11138 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Ti plasmid binary vector pGZ14.1021, complete sequence.
ACCESSION   KP100426
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11138)
  AUTHORS   Zhang Y, Guiltinan MJ, Maximova SN.
  TITLE     Characterization of stearoyl-acyl carrier protein-desaturase gene 
            family from chocolate tree, Theobroma cacao L.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11138)
  AUTHORS   Zhang Y, Guiltinan MJ, Maximova SN.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2014) Plant Sciences, Penn State University, 422 
            Life Sciences Bldg, University Park, PA 16802, USA
REFERENCE   3  (bases 1 to 11138)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11138
                     /organism="Ti plasmid binary vector pGZ14.1021"
                     /mol_type="other DNA"
                     /label=derived from pCAMBIA1300
                     /note="derived from pCAMBIA1300"
                     /db_xref="taxon:1619858"
     regulatory      complement(6..203)
                     /regulatory_class="terminator"
                     /note="35S terminator"
     polyA_signal    10..184
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(210..1400)
                     /codon_start=1
                     /gene="TcSAD1"
                     /product="stearoyl-acyl carrier protein-desaturase 1"
                     /label=TcSAD1
                     /protein_id="AJP67512.1"
                     /translation="MALKLNPITSQSQKLPYFALPPMASLRSPKFFMASTLRSGSKEVE
                     NVKKPFMPPREVHVQVTHSMPPQKIEIFKSLENWAEQNILVHLKPVEKCWQPQDFLPDP
                     ASDGFDEQVKELRERAKEIPDDYFVVLVGDMITEEALPTYQTMLNTLDGVLDETGASLT
                     SWAIWTRAWTAEENRHGDLLNKYLYLSGRVDMRQIEKTIQYLIGSGMDPRTENSPYLGF
                     IYTSFQERATFISHGNTARLAKEHGDFKLAQICGTIASDERRHETAYTKIVEKLFEIDP
                     DGTVLAFADMMRKKISMPAHLMYDGRDDNLFDHFSAVAQRLGVYTAKDYADILEFLVER
                     WKVKELTGLSADGRKAQDYVCGLPPRIRRLEERAQGRAKQAPSIPFSWIFDREVKL"
     gene            complement(210..1400)
                     /gene="TcSAD1"
                     /label=TcSAD1
     regulatory      complement(1412..2230)
                     /regulatory_class="promoter"
                     /gene="35S"
     gene            complement(1412..2230)
                     /gene="35S"
                     /label=35S
     promoter        complement(1481..1526)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(2240..2256)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2459..2483
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    2478..2503
                     /label=right border
                     /note="right border"
     CDS             3783..4412
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4846..5913
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      5979..6173
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6517..6657
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6843..7431)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      6947..7227
                     /label=M13 Ori
                     /note="M13 Ori"
     CDS             complement(7518..8312)
                     /codon_start=1
                     /gene="Kan(R)"
                     /product="Kan(R)"
                     /label=Kan(R)
                     /protein_id="AJP67513.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(7518..8312)
                     /gene="Kan(R)"
                     /label=Kan(R)
     misc_feature    8737..8762
                     /label=left border
                     /note="left border"
     misc_feature    8737..8761
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    8839..9013
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9053..10078)
                     /codon_start=1
                     /gene="Hyg(R)"
                     /product="Hyg(R)"
                     /label=Hyg(R)
                     /protein_id="AJP67514.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     gene            complement(9053..10078)
                     /gene="Hyg(R)"
                     /label=Hyg(R)
     regulatory      10114..10894
                     /regulatory_class="promoter"
                     /gene="CaMV35S"
     gene            10114..10894
                     /gene="CaMV35S"
                     /label=CaMV35S
     promoter        complement(10146..10823)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    11014..11035
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     regulatory      11042..11096
                     /regulatory_class="promoter"
                     /gene="LacZ"
     gene            11042..11096
                     /gene="LacZ"
                     /label=LacZ
     promoter        11050..11080
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11088..11104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11112..11128
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"