LOCUS       Exported                5667 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGV1025 DNA, complete sequence.
ACCESSION   AJ414112
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Broeders S, De Greve H, De Rycke R, Bauw G, Jansens S, Buys L, 
            Smagghe G, Ripoll C, Peumans WJ, Van Damme EJM., Hernalsteens JP.
  TITLE     Leaf and bulb lectins from garlic (Allium sativum L.) are expressed,
            correctly processed and targeted to the cytoplasm in transgenic 
            tobacco plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5667)
  AUTHORS   De Greve H.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2001) De Greve H., Genetische Virologie, Vrije 
            Universiteit Brussel, Paardenstraat 65, Sint-Genesius-Rode, B-1640, 
            BELGIUM
REFERENCE   3  (bases 1 to 5667)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Cloning vector pGV1025"
                     /host="Escherichia coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:176844"
     misc_feature    1..403
                     /label=pUC18
                     /note="pUC18"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      404..1263
                     /regulatory_class="promoter"
                     /note="Cauliflower Mosaic Virus 35S promoter"
     promoter        910..1255
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1294..3105
                     /codon_start=1
                     /gene="gus"
                     /product="beta-glucuronidase"
                     /label=gus
                     /protein_id="CAD10672.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            1294..3105
                     /gene="gus"
                     /label=gus
     3UTR           3176..3431
                     /label=nopaline synthase gene
                     /note="nopaline synthase gene"
     terminator      3176..3428
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3432..5667
                     /label=pUC18
                     /note="pUC18"
     primer_bind     complement(3446..3462)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3470..3486
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3494..3524)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3539..3560
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3848..4436)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4607..5467)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5468..5572)
                     /gene="bla"
                     /label=AmpR promoter