LOCUS       Exported                5199 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  GFP fusion vector pGUG, complete sequence.
ACCESSION   AF515459
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Oka A, Sugisaki H, Takanami M.
  TITLE     Nucleotide sequence of the kanamycin resistance transposon Tn903
  JOURNAL   J. Mol. Biol. 147 (2), 217-226 (1981)
  PUBMED    6270337
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Heim R, Cubitt AB, Tsien RY.
  TITLE     Improved green fluorescence
  JOURNAL   Nature 373 (6516), 663-664 (1995)
  PUBMED    7854443
REFERENCE   3  (bases 1 to 5199)
  AUTHORS   Wach A, Brachat A, Alberti-Segui C, Rebischung C, Philippsen P.
  TITLE     Heterologous HIS3 marker and GFP reporter modules for PCR-targeting 
            in Saccharomyces cerevisiae
  JOURNAL   Yeast 13 (11), 1065-1075 (1997)
  PUBMED    9290211
REFERENCE   4  (bases 1 to 5199)
  AUTHORS   Wendland J, Ayad-Durieux Y, Knechtle P, Rebischung C, Philippsen P.
  TITLE     PCR-based gene targeting in the filamentous fungus Ashbya gossypii
  JOURNAL   Gene 242 (1-2), 381-391 (2000)
  PUBMED    10721732
REFERENCE   5  (bases 1 to 5199)
  AUTHORS   Knechtle P, Dietrich F, Philippsen P.
  TITLE     Maximal Polar Growth Potential Depends on the Polarisome Component 
            AgSpa2 in the Filamentous Fungus Ashbya gossypii
  JOURNAL   Mol. Biol. Cell 14 (10), 4140-4154 (2003)
  PUBMED    12937275
REFERENCE   6  (bases 1 to 5199)
  AUTHORS   Knechtle P, Philippsen P.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2002) Microbiology, Biozentrum, Universitaet 
            Basel, Klingelbergstrasse 70, Basel 4056, Switzerland
REFERENCE   7  (bases 1 to 5199)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="GFP fusion vector pGUG"
                     /mol_type="genomic DNA"
                     /note="for PCR-based C-terminal GFP fusion in Eremothecium 
                     gossypii"
                     /db_xref="taxon:210736"
     promoter        184..202
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    257..292
                     /label=encodes 6x Gly-Ala linker
                     /note="encodes 6x Gly-Ala linker"
     gene            305..1018
                     /gene="GFP"
                     /allele="S65T"
                     /label=GFP
                     /note="encodes C-terminus of green fluorescent protein; C-t
                     of fusion proteins"
     CDS             305..1018
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     regulatory      1027..1268
                     /regulatory_class="terminator"
                     /note="Saccharomyces cerevisiae URA3"
     regulatory      1275..1881
                     /regulatory_class="promoter"
                     /note="Saccharomyces cerevisiae TEF2"
     CDS             1882..2691
                     /codon_start=1
                     /product="kanamycin-resistance protein"
                     /label=kanamycin-resistance protein
                     /protein_id="AAP47193.1"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     regulatory      2692..2966
                     /regulatory_class="terminator"
                     /note="Saccharomyces cerevisiae TEF2"
     promoter        complement(3037..3055)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3313..3901)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4072..4932)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4933..5037)
                     /gene="bla"
                     /label=AmpR promoter