LOCUS       Exported                3267 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGT2, complete sequence.
ACCESSION   EF025088
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Park HK, Zeng C.
  TITLE     Construction of an XcmI-generated T vector bearing green fluorescent
            protein marker for direct cloning of PCR products
  JOURNAL   Anal. Biochem. 360 (1), 144-145 (2007)
  PUBMED    17113027
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Park HK, Zeng C.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2006) Bio. Sci., Univ. of Wisconsin-Milwaukee, 
            3209 N. Maryland Ave., Milwaukee, WI 53211, USA
REFERENCE   3  (bases 1 to 3267)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Cloning vector pGT2"
                     /mol_type="other DNA"
                     /note="derived from cloning vector pGFPTA with addition of 
                     XcmI restriction sites; plasmid available at 
                     www.addgene.org"
                     /db_xref="taxon:404940"
     protein_bind    52..73
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        88..118
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    126..142
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     150..166
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             162..956
                     /codon_start=1
                     /gene="gfpuv4-2aa"
                     /product="GFPuv4-2AA"
                     /label=gfpuv4-2aa
                     /note="2 amino acids were added to the C-terminus of GFPuv4
                     in GenBank Accession Number BAA93576.1"
                     /protein_id="ABI94360.1"
                     /translation="MTMITPSLHACRSTLEDPRVPVEKMSKGEELFTGVVPILVELDGD
                     VNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKR
                     HDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGH
                     KLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
                     HYLSTQSALLKDPNEKRDHMVLLEFVTAAGITHGMDELYKPH"
     gene            162..956
                     /gene="gfpuv4-2aa"
                     /label=gfpuv4-2aa
     misc_feature    214..228
                     /gene="gfpuv4-2aa"
                     /label=XcmI restriction site #1
                     /note="XcmI restriction site #1"
     CDS             234..947
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALLKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    948..962
                     /label=XcmI restriction site #2
                     /note="XcmI restriction site #2"
     promoter        1076..1180
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1181..2041
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="ABI94361.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            1181..2041
                     /gene="bla"
                     /label=bla
     rep_origin      2212..2800
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2986..3126
                     /label=bom
                     /note="basis of mobility region from pBR322"