LOCUS       Exported               11207 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Dual-tagging gene trap vector pGT1 DNA, complete sequence.
ACCESSION   AB028139
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11207)
  AUTHORS   Lukacsovich T, Asztalos Z, Awano W, Baba K, Kondo S, Niwa S, 
            Yamamoto D.
  TITLE     Dual-tagging gene trap of novel genes in Drosophila melanogaster
  JOURNAL   Genetics 157 (2), 727-742 (2001)
  PUBMED    11156992
REFERENCE   2  (bases 1 to 11207)
  AUTHORS   Lukacsovich T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-1999) Tamas Lukacsovich, University of California,
            Irvine, Developmental and Cell Biology; Room 4444, McGaugh Hall, 
            Irvine, California 92697-2300, USA (E-mail:tlukacs@uci.edu, 
            Tel:1-949-824-3226, Fax:1-949-824-3571)
REFERENCE   3  (bases 1 to 11207)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11207
                     /organism="Dual-tagging gene trap vector pGT1"
                     /focus
                     /mol_type="other DNA"
                     /db_xref="taxon:474004"
     source          275..2920
                     /organism="Saccharomyces cerevisiae"
                     /mol_type="other DNA"
                     /db_xref="taxon:4932"
     source          3624..4418
                     /organism="Escherichia coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:562"
     source          5409..8290
                     /organism="Drosophila melanogaster"
                     /mol_type="other DNA"
                     /db_xref="taxon:7227"
     misc_feature    1..233
                     /label=P element 3 end
                     /note="P element 3 end"
     CDS             275..2920
                     /codon_start=1
                     /gene="Gal4"
                     /product="Gal4"
                     /label=Gal4
                     /protein_id="BAA78208.1"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     gene            275..2920
                     /gene="Gal4"
                     /label=Gal4
     CDS             complement(3624..4418)
                     /codon_start=1
                     /gene="neo"
                     /product="neomycine phosphotransferase"
                     /label=neo
                     /protein_id="BAA78209.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            complement(3624..4418)
                     /gene="neo"
                     /label=neo
     promoter        complement(4461..4699)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     CDS             join(5409..5480,5846..6146,6221..6875,6908..7253,7474..7605,
                     7676..8290)
                     /codon_start=1
                     /gene="mini-white"
                     /product="white protein"
                     /label=mini-white
                     /protein_id="BAA78210.1"
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNLIFEIPYHCRVTADASQSCIN
                     QGFGQAKNYGTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRT
                     RGLFCNERHIPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVS
                     PSGMRLLNGQPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVA
                     RVDQVIQELSLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLD
                     SFTAHSVVQVLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDF
                     FSYITLHLNSYPAWVPSVLPTTIRRTFTYRCWPLCPDGRSSPVIGSPRYGDNFAISKVA
                     RDMEQLLATKNLEKPLEQPENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTT
                     MVAILIGLIFLGQQLTQVGVMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARS
                     RLYRCDTYFLGKTIAELPLFLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVST
                     SFGYLISCASSSTSMALSVGPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEG
                     LLINQWADVEPGEISCTSSNTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLA
                     YLALRLRARRKE"
     gene            join(5409..5480,5846..6146,6221..6875,6908..7253,7474..7605,
                     7676..8290)
                     /gene="mini-white"
                     /label=mini-white
     rep_origin      complement(8682..9270)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9441..10301)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10302..10406)
                     /gene="bla"
                     /label=AmpR promoter