LOCUS       Exported                3329 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Binary cloning vector pGreenII-ArrayMCS, complete sequence.
ACCESSION   JN108024
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Shiboleth YM.
  TITLE     pGreenII-ArrayMCS binary cloning vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Shiboleth YM.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2011) Department of Molecular, Cellular, & 
            Developmental Biology, University of Michigan, 830 N. University 
            Ave, 4053 Natural Science Building, Ann Arbor, MI 48109-1048, USA
REFERENCE   3  (bases 1 to 3329)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Binary cloning vector pGreenII-ArrayMCS"
                     /mol_type="other DNA"
                     /PCR_primers="fwd_name: pgreenii679fwd, fwd_seq:
                     gtgctgcaaggcgattaagt, rev_name: pgreenii943rev, rev_seq: 
                     gagcttggcgtaatcatggt"
                     /db_xref="taxon:1107347"
     rep_origin      26..95
                     /label=pSa Ori
                     /note="pSa Ori"
     misc_feature    542..566
                     /label=T-DNA left border (LB)
                     /note="T-DNA left border (LB)"
     misc_feature    542..564
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     regulatory      579..584
                     /regulatory_class="minus_35_signal"
                     /note="Escherichia coli lac promoter RNApol binding site"
     primer_bind     733..749
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        756..774
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    788..914
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        complement(928..946)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(967..983)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    991..1007
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1015..1046)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1061..1082
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    1321..1345
                     /label=T-DNA right border (RB)
                     /note="T-DNA right border (RB)"
     rep_origin      1371..2095
                     /label=pUC Ori
                     /note="pUC Ori"
     rep_origin      complement(1436..2024)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2195..3010)
                     /codon_start=1
                     /product="NptI"
                     /label=NptI
                     /note="kanamycin resistance protein"
                     /protein_id="AER45487.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3301..407
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"