LOCUS       Exported                3255 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Binary cloning vector pGreenII-35S, complete sequence.
ACCESSION   KX826944
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Tuo D.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2016) Papaya Biotechnology Laboratory, Institute 
            of Tropical Bioscience and Biotechnology, Chinese Academy of 
            Tropical Agricultural Science, 4 Xueyuan Road, Haikou, Hainan, 
            Haikou, Hannan Province 571101, China
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Binary cloning vector pGreenII-35S"
                     /mol_type="other DNA"
                     /db_xref="taxon:1966393"
     rep_origin      join(1..525,3222..3255)
                     /label=pSa Ori
                     /note="pSa Ori"
     misc_feature    join(1..568,1244..3255)
                     /label=pGreenII 0000 vector sequence
                     /note="pGreenII 0000 vector sequence"
     misc_feature    542..566
                     /label=T-DNA left border (LB)
                     /note="T-DNA left border (LB)"
     misc_feature    542..564
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     regulatory      complement(569..798)
                     /regulatory_class="terminator"
                     /note="CaMV polyA terminator"
     polyA_signal    572..748
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    797..843
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        complement(841..1186)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      complement(844..1243)
                     /regulatory_class="promoter"
                     /note="CaMV 35S promoter"
     misc_feature    1247..1271
                     /label=T-DNA right border (RB)
                     /note="T-DNA right border (RB)"
     rep_origin      1297..2021
                     /label=pUC-Ori
                     /note="pUC-Ori"
     rep_origin      complement(1362..1950)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2121..2936)
                     /codon_start=1
                     /gene="nptI"
                     /product="NptI"
                     /label=nptI
                     /note="kanamycin resistance protein"
                     /protein_id="AQX83314.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(2121..2936)
                     /gene="nptI"
                     /label=nptI
     rep_origin      3227..407
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"