LOCUS       Exported               10730 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    p58sheLL
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10730)
  AUTHORS   Kondo K, Ishii Y, Ochi H, Matsumoto T, Yoshikawa H, Kanda T
  TITLE     Neutralization of HPV16, 18, 31, and 58 pseudovirions with antisera 
            induced by immunizing rabbits with synthetic peptides representing 
            segments of the HPV16 minor capsid protein L2 surface region.
  JOURNAL   Virology. 2007 Feb 20;358(2):266-72. Epub 2006 Sep 28.
  PUBMED    17010405
REFERENCE   2  (bases 1 to 10730)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2007-02-20"; volume: "358(2)"; pages: "266-72. Epub 2006 Sep 
            28"
FEATURES             Location/Qualifiers
     source          1..10730
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    3667..4255
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3720..3740)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4138..4149)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4292..4309)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4298..4522
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4568..4996
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4655..4674)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4865..4886
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5005..5025)
                     /label=pBABE 3
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             6954..7322
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="KLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVT
                     LFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGREF
                     ALRDPAGNCVHFVAEEQD"
     polyA_signal    7452..7573
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7489..7508)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7543..7562
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7622..7638)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7622..7638)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7635..7657)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7646..7662
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7670..7700)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7715..7736
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7853..7870)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8024..8609)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8104..8123)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8780..9640)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9403..9422
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9641..9745)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9820..9839)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10011..10390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10391..10594
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10545..10565
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    10596..10614
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10617..10635
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10645..10669
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"