LOCUS       Exported                5647 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGR-ermB, complete sequence.
ACCESSION   MF948287
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Hullahalli K, Rodrigues M, Nguyen U, Palmer K.
  TITLE     A semi-lethal CRISPR-Cas system permits DNA acquisition in 
            Enterococcus faecalis
  JOURNAL   bioRxivorg (2018) In press
REFERENCE   2  (bases 1 to 5647)
  AUTHORS   Hullahalli K.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-SEP-2017) Biological Sciences, The University of Texas
            at Dallas, 800 W Campbell Rd., Richardson, TX 75080, USA
REFERENCE   3  (bases 1 to 5647)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Cloning vector pGR-ermB"
                     /mol_type="other DNA"
                     /db_xref="taxon:2058393"
     misc_feature    complement(567..602)
                     /label=CRISPR1 repeat
                     /note="CRISPR1 repeat"
     misc_feature    complement(603..632)
                     /label=ermB spacer
                     /note="ermB spacer"
     regulatory      complement(633..682)
                     /regulatory_class="promoter"
                     /note="PbacA"
     CDS             complement(1567..1578)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2190..2888
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein repA"
                     /label=repA
                     /protein_id="AUB51326.1"
                     /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE
                     KKDKDTWNSSDVIRNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD
                     YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRKLKNLLLDIV
                     DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK
                     VLDAETGEIK"
     gene            2190..2888
                     /gene="repA"
                     /label=repA
     rep_origin      3063..3332
                     /label=oriT
                     /note="oriT"
     CDS             complement(3655..4302)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /protein_id="AUB51324.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKKK
                     GYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWTES
                     DKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNSSFL
                     LPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(3655..4302)
                     /gene="cat"
                     /label=cat
     CDS             complement(4584..5630)
                     /codon_start=1
                     /gene="pheS*"
                     /product="phenylalanyl-tRNA synthetase subunit alpha*"
                     /label=pheS*
                     /note="counterselectable with para-chloro-phenylalanine"
                     /protein_id="AUB51325.1"
                     /translation="MTLQAQLEALRDNTLKEIAQVATLKELNQIRVETLGKKGPITEVL
                     RGMKNLSPEERPVVGGFANEIRDLLTEAIEARKVVLENEALNAALKEESLDVTLPGKQM
                     PQGTRHILTQVMEEIEDIFLGMGYQVVEGYEVESDHYNFERMNLPKDHPARDMQDTFYI
                     SDEMLIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEGLVVD
                     KNVTMGDLKGTLEVMMKKMFGEDRKIRLRPSYFPFTEPSVEVDVSCFKCGGAGCNVCKH
                     TGWIEILGAGMVHPDVLQMSGIDPTEYSGFGFGLGPDRVAMLRYGVNDIRNFYQNDLRF
                     LNQFKVKE"
     gene            complement(4584..5630)
                     /gene="pheS*"
                     /label=pheS*