LOCUS       Exported                6376 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Suicide vector pGPI-SceI-XErm, complete sequence.
ACCESSION   KY368390
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Yero D, Ferrer-Navarro M, Costenaro L, Planell R, Conchillo-Sole O, 
            Sole M, Roca I, Gibert I, Daura X.
  TITLE     A transport system associated with the intrinsic antimicrobial 
            multiresistance in Pseudomonas aeruginosa
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6376)
  AUTHORS   Yero D, Gibert I.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2016) Genetica Molecular Bacteriana, Institut de 
            Biotecnologia i de Biomedicina (IBB), Campus UAB, Bellaterra, 
            Barcelona 08191, Spain
REFERENCE   3  (bases 1 to 6376)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Suicide vector pGPI-SceI-XErm"
                     /mol_type="other DNA"
                     /db_xref="taxon:2054240"
     oriT            625..734
                     /note="incP origin of transfer"
     CDS             767..1138
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     CDS             872..1138
                     /codon_start=1
                     /gene="traJ"
                     /product="TraJ"
                     /label=traJ
                     /protein_id="ATW75325.1"
                     /translation="MSLSAYLLAVGQGYKITGVVDYEHVRELARINGDLGRLGGLLKLW
                     LTDDPRTARFGDATILALLAKIEEKQDELGKVMMGVVRPRAEP"
     gene            872..1138
                     /gene="traJ"
                     /label=traJ
     CDS             complement(1101..1526)
                     /codon_start=1
                     /gene="traI"
                     /product="TraI"
                     /label=traI
                     /protein_id="ATW75324.1"
                     /translation="MNVQVVGVVMHGTDALMLGEAKPSADAVLNRAQRLRVGLLSRAEA
                     NQQVIGLVGLGTGVAVLGRHHLGHDSGQGVCLAVRDAHVTQALGLALLVGDVLHQLREV
                     ALLDGAHGDVLGNHAHPPAVLAAKKVMALPSGGPRPS"
     gene            complement(1101..1526)
                     /gene="traI"
                     /label=traI
     rep_origin      1756..2147
     rep_origin      1756..2144
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     misc_feature    2149..2177
                     /label=multiple cloning site 1
                     /note="multiple cloning site 1"
     promoter        2193..2221
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     CDS             2253..3176
                     /codon_start=1
                     /gene="xylE"
                     /product="XylE reporter protein"
                     /label=xylE
                     /protein_id="ATW75326.1"
                     /translation="MNKGVMRPGHVQLRVLDMSKALEHYVELLGLIEMDRDDQGRVYLK
                     AWTEVDKFSLVLREADEPGMDFMGFKVVDEDALRQLERDLMAYGCAVEQLPAGELNSCG
                     RRVRFQAPSGHHFELYADKEYTGKWGLNDVNPEAWPRDLKGMAAVRFDHALMYGDELPA
                     TYDLFTKVLGFYLAEQVLDENGTRVAQFLSLSTKAHDVAFIHHPEKGRLHHVSFHLETW
                     EDLLRAADLISMTDTSIDIGPTRHGLTHGKTIYFFDPSGNRNEVFCGGDYNYPDHKPVT
                     WTTDQLGKAIFYHDRILNERFMTVLT"
     gene            2253..3176
                     /gene="xylE"
                     /label=xylE
     misc_feature    3182..3215
                     /label=multiple cloning site 2
                     /note="multiple cloning site 2"
     CDS             3939..4175
                     /codon_start=1
                     /gene="dhfr"
                     /product="DHFR trimethoprim resistance protein"
                     /label=dhfr
                     /note="promoterless gene"
                     /protein_id="ATW75327.1"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     gene            3939..4175
                     /gene="dhfr"
                     /label=dhfr
     misc_feature    4178..4195
                     /label=I-SceI recognition site
                     /note="I-SceI recognition site"
     CDS             4582..5319
                     /codon_start=1
                     /gene="ermB"
                     /product="Erythromycin resistance protein"
                     /label=ermB
                     /note="rRNA adenine N-6-methyltransferase"
                     /protein_id="ATW75323.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT
                     KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYH
                     LSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFH
                     PKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNN
                     LSTVTYEQVLSIFNSYLLFNGRK"
     gene            4582..5319
                     /gene="ermB"
                     /label=ermB
                     /note="rRNA adenine N-6-methyltransferase"