LOCUS       Exported                4925 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Mutagenesis vector pGPI-SceI-2, complete sequence.
ACCESSION   KC409642
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Hamad MA, Di Lorenzo F, Molinaro A, Valvano MA.
  TITLE     Aminoarabinose is essential for lipopolysaccharide export and 
            intrinsic antimicrobial peptide resistance in Burkholderia 
            cenocepacia(dagger)
  JOURNAL   Mol. Microbiol. 85 (5), 962-974 (2012)
  PUBMED    22742453
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Hamad MA, Di Lorenzo F, Molinaro A, Valvano MA.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2012) Microbiology and Immunology, Western 
            University, 1151 Richmond Street North, London, Ontario N6A 5C1, 
            Canada
REFERENCE   3  (bases 1 to 4925)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Mutagenesis vector pGPI-SceI-2"
                     /mol_type="other DNA"
                     /db_xref="taxon:1276081"
     oriT            626..735
                     /note="incP origin of transfer"
     CDS             768..1139
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     CDS             873..1139
                     /codon_start=1
                     /product="TraJ"
                     /label=TraJ
                     /protein_id="AGC79937.1"
                     /translation="MSLSAYLLAVGQGYKITGVVDYEHVRELARINGDLGRLGGLLKLW
                     LTDDPRTARFGDATILALLAKIEEKQDELGKVMMGVVRPRAEP"
     CDS             complement(1102..1527)
                     /codon_start=1
                     /product="TraI"
                     /label=TraI
                     /protein_id="AGC79936.1"
                     /translation="MNVQVVGVVMHGTDALMLGEAKPSADAVLNRAQRLRVGLLSRAEA
                     NQQVIGLVGLGTGVAVLGRHHLGHDSGQGVCLAVRDAHVTQALGLALLVGDVLHQLREV
                     ALLDGAHGDVLGNHAHPPAVLAAKKVMALPSGGPRPS"
     rep_origin      1757..2148
     rep_origin      1757..2145
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     misc_feature    2149..2175
                     /label=multiple cloning site 1
                     /note="multiple cloning site 1"
     misc_feature    2388..2421
                     /label=multiple cloning site 2
                     /note="multiple cloning site 2"
     promoter        3150..3178
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             3504..3740
                     /codon_start=1
                     /product="DHFR trimethoprim resistance protein"
                     /label=DHFR trimethoprim resistance protein
                     /protein_id="AGC79935.1"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     misc_feature    3743..3760
                     /label=I SceI recognition site
                     /note="I SceI recognition site"