LOCUS       Exported                4989 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGloSensor-10F[TEV], complete sequence.
ACCESSION   EU693493
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Wigdal SS, Frackman S, Slater M.
  TITLE     pGloSensor-10F[TEV] Control Plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Wigdal SS, Frackman S, Slater M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAY-2008) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 4989)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Cloning vector pGloSensor-10F[TEV]"
                     /mol_type="other DNA"
                     /db_xref="taxon:543370"
     regulatory      23..42
                     /regulatory_class="promoter"
                     /note="SP6 RNA polymerase promoter"
     promoter        23..41
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      46..63
                     /regulatory_class="promoter"
                     /note="T7 RNA polymerase promoter"
     promoter        46..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5UTR           65..201
                     /label=Barley yellow dwarf virus 5 untranslated region
                     /note="Barley yellow dwarf virus 5 untranslated region"
     CDS             211..1875
                     /codon_start=1
                     /product="GloSensor-10F[TEV]"
                     /label=GloSensor-10F[TEV]
                     /note="circularly permuted form of firefly luciferase with 
                     a TEV protease recognition sequence"
                     /protein_id="ACE95898.1"
                     /translation="MDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSL
                     QDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGI
                     RQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGP
                     MIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAEL
                     ESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLR
                     GGVVFVDEVPKGLTGKLDARKIREILIKAKKASENLYFQSGSAKNIKKGPAPFYPLEDG
                     TAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIV
                     VCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILN
                     VQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMN
                     SSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPV"
     CDS             1153..1173
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     misc_feature    1896..2002
                     /label=Barley yellow dwarf virus 3 translation enhancer
                     /note="Barley yellow dwarf virus 3 translation enhancer"
     regulatory      2107..2154
                     /regulatory_class="terminator"
                     /note="T7 terminator"
     terminator      2107..2154
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        2383..2487
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2488..3348
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="confers ampicillin resistance"
                     /protein_id="ACE95899.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3503..3539
                     /label=origin of replication
                     /note="origin of replication"
     rep_origin      complement(3506..4094)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_recomb     4210..4495
                     /note="cer site; site for Escherichia coli XerCD 
                     recombinase"
     misc_feature    4210..4493
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     regulatory      4546..4947
                     /regulatory_class="terminator"
                     /note="rrnB transcription terminator"
     terminator      4723..4809
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4901..4928
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"