LOCUS       Exported                6248 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pGL3-3UTR, complete sequence.
ACCESSION   JN542721
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Bi Y, Qiao X, Hua Z, Zhang L, Liu X, Li L, Hua W, Xiao H, Zhou J, 
            Wei Q, Zheng X.
  TITLE     An asymmetric PCR-based, reliable and rapid single-tube native DNA 
            engineering strategy
  JOURNAL   BMC Biotechnol. 12, 39 (2012)
  PUBMED    22768962
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Bi Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2011) Biotechnology Group, Institute of Animal 
            Science and Veterinary, Hubei Academy of Agricultural Science, No. 
            1, Nanhu Yaoyuan, Hongshan District, Wuhan, Hubei 430064, P. R. 
            China
REFERENCE   3  (bases 1 to 6248)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Reporter vector pGL3-3UTR"
                     /mol_type="other DNA"
                     /db_xref="taxon:1219474"
     promoter        48..244
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      95..230
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      274..283
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             280..1932
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     regulatory      1988..3433
                     /regulatory_class="terminator"
     regulatory      2170..2175
                     /regulatory_class="polyA_signal_sequence"
     regulatory      2584..2589
                     /regulatory_class="polyA_signal_sequence"
     regulatory      2908..2913
                     /regulatory_class="polyA_signal_sequence"
     regulatory      3269..3274
                     /regulatory_class="polyA_signal_sequence"
     rep_origin      complement(3751..4339)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4510..5370)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5371..5475)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5502..5957
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     polyA_signal    6088..6136
                     /note="synthetic polyadenylation signal"
     misc_feature    6150..6241
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"