LOCUS       Exported                3284 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression shuttle vector pGJ103, complete sequence.
ACCESSION   KU307276
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ming-Ming Y, Wei-Wei Z, Xi-Feng Z, Pei-Lin C.
  TITLE     Construction and characterization of a novel maltose inducible 
            expression vector in Bacillus subtilis
  JOURNAL   Biotechnol. Lett. 28 (21), 1713-1718 (2006)
  PUBMED    17001500
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Yang MM, Zhang WW.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2015) College of Animal Sciences and Technology, 
            Northwest A&F University, Xinong Road, Yangling, Shanxi 712100, P. 
            R. China
REFERENCE   3  (bases 1 to 3284)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Expression shuttle vector pGJ103"
                     /mol_type="other DNA"
                     /country="China"
                     /note="Escherichia coli-Bacillus subtilis shuttle 
                     expression vector"
                     /db_xref="taxon:1849381"
     CDS             1193..1840
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /protein_id="ANE31721.1"
                     /translation="MTFNIIKLENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKKK
                     GYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWTES
                     DNNFTSFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNSNFL
                     LPIITIGKFYSENNKIYIPVALQLHHAVCDGYHASLFMNEFQDIIHKVDDWI"
     gene            1193..1840
                     /gene="cat"
                     /label=cat
     rep_origin      complement(2003..2591)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2643..2659
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2693..2709)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"