LOCUS       Exported                5147 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Wheat transformation vector pghp8.1, complete sequence.
ACCESSION   HM352557
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Gil-Humanes J, Piston F, Tollefsen S, Sollid LM, Barro F.
  TITLE     Effective shutdown in the expression of celiac disease-related wheat
            gliadin T-cell epitopes by RNA interference
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 107 (39), 17023-17028 (2010)
  PUBMED    20829492
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   Gil-Humanes J, Piston F, Tollefsen S, Aouni R, Sollid LM, Barro F.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2010) Mejora Genetica Vegetal, Instituto de 
            Agricultura Sostenible. CSIC, Alameda del Obispo. s/n, Cordoba, 
            Cordoba 14080, Spain
REFERENCE   3  (bases 1 to 5147)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Wheat transformation vector pghp8.1"
                     /mol_type="other DNA"
                     /db_xref="taxon:886297"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      402..1237
                     /regulatory_class="promoter"
                     /note="D-hordein promoter"
     misc_feature    1244..1413
                     /note="pg8.1 sense; gamma-gliadin antisense"
     intron          1414..2437
                     /note="maize ubiquitin intron 1; Ubi 1 intron"
     misc_feature    2444..2612
                     /note="pg8.1 antisense; gamma-gliadin antisense"
     terminator      2635..2887
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      2636..2907
                     /regulatory_class="terminator"
                     /note="Agrobacterium tumefaciens nopaline synthase 
                     terminator; nos terminator"
     primer_bind     complement(2926..2942)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2950..2966
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2974..3004)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3019..3040
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3328..3916)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4087..4947)
                     /codon_start=1
                     /product="bla gene-Ap(r)"
                     /label=bla gene-Ap
                     /note="ampicilin resistant gene"
                     /protein_id="ADN83669.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4948..5052)
                     /gene="bla"
                     /label=AmpR promoter