LOCUS       Exported                3933 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGGY003, complete sequence.
ACCESSION   KF718975
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Forner J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies, 
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230, 
            Heidelberg D-69221, Germany
REFERENCE   3  (bases 1 to 3933)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Cloning vector pGGY003"
                     /mol_type="other DNA"
                     /db_xref="taxon:1439702"
     rep_origin      26..515
                     /note="for replication in Agrobacterium tumefaciens; pSa 
                     origin of replication"
     misc_feature    529..560
                     /label=T-DNA left border
                     /note="T-DNA left border"
     misc_feature    534..556
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     misc_feature    complement(607..612)
                     /label=BsaI recognition site
                     /note="BsaI recognition site"
     CDS             743..1402
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /protein_id="AHE38479.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            743..1402
                     /gene="cat"
                     /label=cat
     CDS             1421..1726
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB"
                     /label=ccdB
                     /protein_id="AHE38480.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            1421..1726
                     /gene="ccdB"
                     /label=ccdB
     CDS             1745..1996
                     /codon_start=1
                     /gene="lacZ alpha"
                     /product="beta-galactosidase alpha peptide"
                     /label=lacZ alpha
                     /protein_id="AHE38481.1"
                     /translation="MLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQ
                     LRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICSDAA"
     gene            1745..1996
                     /gene="lacZ alpha"
                     /label=lacZ alpha
     misc_feature    2040..2045
                     /label=BsaI recognition site
                     /note="BsaI recognition site"
     misc_feature    2130..2182
                     /label=T-DNA right border
                     /note="T-DNA right border"
     misc_feature    2133..2157
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(2248..2836)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3081..3614)
                     /codon_start=1
                     /gene="AAC(3)-I"
                     /product="aminoglycoside N(3)-acetyltransferase I"
                     /label=AAC(3)-I
                     /protein_id="AHE38478.1"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQARS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     gene            complement(3081..3614)
                     /gene="AAC(3)-I"
                     /label=AAC(3)-I
     rep_origin      3905..407
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"