LOCUS       Exported                4869 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGGF004, complete sequence.
ACCESSION   KF719010
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Forner J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies, 
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230, 
            Heidelberg D-69221, Germany
REFERENCE   3  (bases 1 to 4869)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Cloning vector pGGF004"
                     /mol_type="other DNA"
                     /db_xref="taxon:1439690"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             201..1061
                     /codon_start=1
                     /gene="bla"
                     /product="beta lactamase"
                     /label=bla
                     /protein_id="AHE38542.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            201..1061
                     /gene="bla"
                     /label=bla
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    2241..2246
                     /label=BsaI recognition site
                     /note="BsaI recognition site"
     misc_feature    2252..4434
                     /label=p35S:D-alaR:t35S
                     /note="p35S:D-alaR:t35S"
     promoter        2747..3092
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3111..4217
                     /codon_start=1
                     /gene="dao1"
                     /product="D-amino acid oxidase"
                     /label=dao1
                     /note="from Rhodosporidium toruloides"
                     /protein_id="AHE38543.1"
                     /translation="MHSQKRVVVLGSGVIGLSSALILARKGYSVHILARDLPEDVSSQT
                     FASPWAGANWTPFMTLTDGPRQAKWEESTFKKWVELVPTGHAMWLKGTRRFAQNEDGLL
                     GHWYKDITPNYRPLPSSECPPGAIGVTYDTLSVHAPKYCQYLARELQKLGATFERRTVT
                     SLEQAFDGADLVVNATGLGAKSIAGIDDQAAEPIRGQTVLVKSPCKRCTMDSSDPASPA
                     YIIPRPGGEVICGGTYGVGDWDLSVNPETVQRILKHCLRLDPTISSDGTIEGIEVLRHN
                     VGLRPARRGGPRVEAERIVLPLDRTKSPLSLGRGSARAAKEKEVTLVHAYGFSSAGYQQ
                     SWGAAEDVAQLVDEAFQRYHGAARESKL"
     gene            3111..4217
                     /gene="dao1"
                     /label=dao1
     misc_feature    complement(4440..4445)
                     /label=BsaI recognition site
                     /note="BsaI recognition site"
     promoter        complement(4456..4474)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"