LOCUS       Exported                3249 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGFPTA DNA, complete sequence.
ACCESSION   AB038146
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ito Y, Suzuki M, Husimi Y.
  TITLE     A T-extended vector using a green fluorescent protein as an 
            indicator
  JOURNAL   Gene 245 (1), 59-63 (2000)
  PUBMED    10713445
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Ito Y, Suzuki M, Husimi Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2000) Yuzuru Husimi, Saitama University, 
            Deptartment of Functional Materials Science; 255 Shimo-ookubo, 
            Urawa, Saitama 338-8750, Japan 
            (E-mail:ito@evolve.fms.saitama-u.ac.jp, Tel:+81-48-858-3531, 
            Fax:+81-48-858-3531)
REFERENCE   3  (bases 1 to 3249)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Cloning vector pGFPTA"
                     /focus
                     /mol_type="other DNA"
                     /db_xref="taxon:116587"
     source          1..956
                     /organism="Cloning vector pGFPuv"
                     /mol_type="other DNA"
                     /db_xref="taxon:49914"
     source          957..3249
                     /organism="Cloning vector pBR322"
                     /mol_type="other DNA"
                     /db_xref="taxon:47470"
     protein_bind    52..73
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        88..118
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    126..142
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     150..166
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             162..950
                     /codon_start=1
                     /gene="gfpuv4"
                     /product="GFPuv4"
                     /label=gfpuv4
                     /protein_id="BAA93576.1"
                     /translation="MTMITPSLHACRSTLEDPRVPVEKMSKGEELFTGVVPILVELDGD
                     VNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKR
                     HDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGH
                     KLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
                     HYLSTQSALLKDPNEKRDHMVLLEFVTAAGITHGMDELYK"
     gene            162..950
                     /gene="gfpuv4"
                     /label=gfpuv4
     CDS             234..947
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALLKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    952..957
                     /label=EcoRI/PvuII restriction site
                     /note="EcoRI/PvuII restriction site"
     promoter        1058..1162
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1163..2023
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="BAA93577.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            1163..2023
                     /gene="bla"
                     /label=bla
     rep_origin      2194..2782
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2968..3108
                     /label=bom
                     /note="basis of mobility region from pBR322"