LOCUS       Exported                3419 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGFPm-T, complete sequence.
ACCESSION   EU341596
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Luo K, Harding SA, Tsai CJ.
  TITLE     A modified T-vector for simplified assembly of hairpin RNAi 
            constructs
  JOURNAL   Biotechnol. Lett. 30 (7), 1271-1274 (2008)
  PUBMED    18317701
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Luo K-M., Harding SA, Tsai C-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2007) School of Forest Resources and Environmental
            Science, Michigan Technological University, 1400 Townsend Drive, 
            Houghton, MI 49931-1295, USA
REFERENCE   3  (bases 1 to 3419)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Cloning vector pGFPm-T"
                     /mol_type="other DNA"
                     /db_xref="taxon:491879"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        266..283
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    310..373
                     /note="multiple cloning site; includes SwaI, BamHI, XcmI, 
                     XcmI, SpeI and AscI"
     promoter        complement(411..429)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             450..1166
                     /codon_start=1
                     /gene="gfpuv4"
                     /product="green fluorescent protein variant"
                     /label=gfpuv4
                     /note="GFP; BamHI site in gfpuv4 deleted"
                     /protein_id="ABY58001.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     gene            450..1166
                     /gene="gfpuv4"
                     /label=gfpuv4
     promoter        1514..1618
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1619..2479
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="ABY58002.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            1619..2479
                     /gene="bla"
                     /label=bla
     rep_origin      2650..3238
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"