LOCUS       Exported               10764 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Transformation vector pGFPBSDZ-SW2, complete sequence.
ACCESSION   KF724860
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10764)
  AUTHORS   Ding H, Gong S, Tian Y, Yang Z, Brunham R, Zhong G.
  TITLE     Transformation of Sexually Transmitted Infection-Causing Serovars of
            Chlamydia trachomatis Using Blasticidin for Selection
  JOURNAL   PLoS ONE 8 (11), E80534 (2013)
  PUBMED    24303023
REFERENCE   2  (bases 1 to 10764)
  AUTHORS   Zhong G, Gong S, Ding H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-OCT-2013) Microbiology and Immunology, University of 
            Texas Health Science Center San Antonio, 7703 Floyd Curl Drive, San 
            Antonio, TX 78229, USA
REFERENCE   3  (bases 1 to 10764)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10764
                     /organism="Transformation vector pGFPBSDZ-SW2"
                     /lab_host="Chlamydia trachomatis bv. Trachoma sv. E str. 
                     SW2"
                     /mol_type="other DNA"
                     /db_xref="taxon:1439520"
     rep_origin      1..88
                     /note="putative; contains 4 copies of a 22bp repeat"
     CDS             165..680
                     /codon_start=1
                     /locus_tag="PSW2_1"
                     /product="putative virulence integrase pGP7-D"
                     /label=PSW2_1
                     /protein_id="AHD24922.1"
                     /translation="MGSMAFHKSRLFLTFGDASEIWLSTLSYLTRKNYASGINFLVSLE
                     ILDLSETLIKAISLDHSESLFKIKSLDVFNGKVVSEASKQARAACYISFTKFLYRLTKG
                     YIKPAIPLKDFGNTTFFKIRDKIKTESISKQEWTVFFEALRIVNYRDYLIGKLIVQGIP
                     GYRARIHR"
     gene            165..680
                     /locus_tag="PSW2_1"
                     /label=PSW2_1
     promoter        complement(694..712)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        1058..1162
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1163..1537
                     /codon_start=1
                     /gene="zeoR"
                     /product="zeocin resistance protein"
                     /label=zeoR
                     /protein_id="AHD24923.1"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     gene            1163..1537
                     /gene="zeoR"
                     /label=zeoR
     rep_origin      1708..2296
                     /label=pUC ori
                     /note="pUC ori"
     promoter        2554..2572
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3079..4200
                     /codon_start=1
                     /gene="GFP-BSD"
                     /product="green fluorescent protein-blasticidin S deaminase
                     fusion protein"
                     /label=GFP-BSD
                     /protein_id="AHD24924.1"
                     /translation="MVDPRVPMSKGEALFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERT
                     IFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADK
                     QKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKR
                     DHMVLLEFVTAAGITHGMDQAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDG
                     RIFTGVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLD
                     LHPGIKAIVKDSDGQPTAVGIRELLPSGYVWEG"
     gene            3079..4200
                     /gene="GFP-BSD"
                     /label=GFP-BSD
     CDS             3805..4200
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(4351..5343)
                     /codon_start=1
                     /locus_tag="PSW2_2"
                     /product="virulence integrase pGP8-D"
                     /label=PSW2_2
                     /protein_id="AHD24925.1"
                     /translation="MGKGILSLQQEMSLEYSEKSYQEVLKIRQESYWKRMKSFSLFEVI
                     MHWTASLNKHTCRSYRGSFLSLEKIGLLSLDMNLQEFSLLNHNLILDAIKKVSSAKTSW
                     TEGTKQVRAASYISLTRFLNRMTQGIVAIAQPSKQENSRTFFKTREIVKTDAMNSLQTA
                     SFLKELKKINARDWLIAQTMLQGGKRSSEVLSLEISQICFQQATISFSQLKNRQTEKRI
                     IITYPQKFMHFLQEYIGQRRGFVFVTRSGKMVGLRQIARTFSQAGLQAAIPFKITPHVL
                     RATAVTEYKRLGCSDSDIMKVTGHATAKMIFAYDKSSREDNASKKLALI"
     gene            complement(4351..5343)
                     /locus_tag="PSW2_2"
                     /label=PSW2_2
     CDS             5486..6841
                     /codon_start=1
                     /locus_tag="PSW2_3"
                     /product="putative DnaB family helicase pGP1-D"
                     /label=PSW2_3
                     /protein_id="AHD24926.1"
                     /translation="MKTRSEIENRMQDIEYALLGKALIFEDSTEYILRQLANYEFKCSH
                     HKNIFIVFKYLKDNGLPITVDSAWEELLRRRIKDMDKSYLGLMLHDALSNDKLRSVSHT
                     VFLDDLSVCSAEENLSNFIFRSFNEYNENPLRRSPFLLLERIKGRLDSAIAKTFSIRSA
                     RGRSIYDIFSQSEIGVLARIKKRRVAFSENQNSFFDGFPTGYKDIDDKGVILAKGNFVI
                     IAARPSIGKTALAIDMAINLAVTQQRRVGFLSLEMSAGQIVERIIANLTGISGEKLQRG
                     DLSKEELFRVEEAGETVRESHFYICSDSQYKLNLIANQIRLLRKEDRVDVIFIDYLQLI
                     NSSVGENRQNEIADISRTLRGLASELNIPIVCLSQLSRKVEDRANKVPMLSDLRDSGQI
                     EQDADVILFINRKESSSNCEITVGKNRHGSVFSSVLHFDPKISKFSAIKKVW"
     gene            5486..6841
                     /locus_tag="PSW2_3"
                     /label=PSW2_3
     CDS             6835..7899
                     /codon_start=1
                     /locus_tag="PSW2_4"
                     /product="putative virulence protein pGP2-D"
                     /label=PSW2_4
                     /protein_id="AHD24927.1"
                     /translation="MVNYSNCHFIKSPIHLENQKFGRRPGQSIKISPKLAQNGMVEVIG
                     LDFLSSHYHALAAIQRLLTATNYKGNTKGVVLSRESNSFQFEGWIPRIRFTKTEFLEAY
                     GVKRYKTSRNKYEFSGKEAETALEALYHLGHQPFLIVATRTRWTNGTQIVDRYQTLSPI
                     IRIYEGWEGLTDEENIDIDLTPFNSPPTRKHKGFVVEPCPILVDQIESYFVIKPANVYQ
                     EIKMRFPNASKYAYTFIDWVITAAAKKRRKLTKDNSWPENLLLNVNVKSLAYILRMNRY
                     ICTRNWKKIELAIDKCIEIAIKLGWLSRRKRIEFLDSSKLSKKEILYLNKERFEEITKK
                     SKEQMEQLEQESIN"
     gene            6835..7899
                     /locus_tag="PSW2_4"
                     /label=PSW2_4
     CDS             7961..8755
                     /codon_start=1
                     /locus_tag="PSW2_5"
                     /product="putative virulence protein pGP3-D"
                     /label=PSW2_5
                     /protein_id="AHD24928.1"
                     /translation="MGNSGFYLYNTENCVFADNIKVGQMTEPLKDQQIILGTTSTPVAA
                     KMTASDGISLTVSNNSSTNASITIGLDAEKAYQLILEKLGDQILDGIADTIVDSTVQDI
                     LDKIKTDPSLGLLKAFNNFPITNKIQCNGLFTPSNIETLLGGTEIGKFTVTPKSSGSMF
                     LVSADIIASRMEGGVVLALVREGDSKPCAISYGYSSGIPNLCSLRTSITNTGLTPTTYS
                     LRVGGLESGVVWVNALSNGNDILGITNTSNVSFLEVIPQTNA"
     gene            7961..8755
                     /locus_tag="PSW2_5"
                     /label=PSW2_5
     CDS             8825..9133
                     /codon_start=1
                     /locus_tag="PSW2_6"
                     /product="putative virulence protein pGP4-D"
                     /label=PSW2_6
                     /protein_id="AHD24929.1"
                     /translation="MQNKRKVRDDFIKIVKDVKKDFPELDLKIRVNKEKVTFLNSPLEL
                     YHKSVSLILGLLQQIENSLGLFPDSPVLEKLEDNSLKLKKALIMLILSRKDMFSKAE"
     gene            8825..9133
                     /locus_tag="PSW2_6"
                     /label=PSW2_6
     CDS             9161..9955
                     /codon_start=1
                     /locus_tag="PSW2_7"
                     /product="putative ParA family partitioning protein pGP5-D"
                     /label=PSW2_7
                     /protein_id="AHD24930.1"
                     /translation="MHTLVFCSFKGGTGKTTLSLNVGCNLAQFLGKKVLLADLDPQSNL
                     SSGLGASVRSDQKGLHDIVYTSNDLKSIICETKKDSVDLIPASFSSEQFRELDIHRGPS
                     NNLKLFLNEYCAPFYDICIIDTPPSLGGLTKEAFVAGDKLIACLTPEPFSILGLQKIRE
                     FLSSVGKPEEEHILGIALSFWDDRNSTNQMYIDIIESIYKNKLFSTKIRRDISLSRSLL
                     KEDSVANVYPNSRAAEDILKLTHEIANILHIEYERDYSQRTT"
     gene            9161..9955
                     /locus_tag="PSW2_7"
                     /label=PSW2_7
     CDS             9952..10695
                     /codon_start=1
                     /locus_tag="PSW2_8"
                     /product="putative virulence protein pGP6-D"
                     /label=PSW2_8
                     /protein_id="AHD24931.1"
                     /translation="MNKLKKEADVFFKKNQTAASLDFKKTLPSIELFSATLNSEESQSL
                     DRLFLSESQNYSDEEFYQEDILAVKLLTGQIKSIQKQHVLLLGEKIYNARKILSKDHFS
                     STTFSSWIELVFRTKSSAYNALAYYELFINLPNQTLQKEFQSIPYKSAYILAARKGDLK
                     TKVDVIGKVCGMSNSSAIRVLDQFLPSSRNKDVRETIDKSDSEKNRQLSDFLIEILRIM
                     CSGVSLSSYNENLLQQLFELFKQKS"
     gene            9952..10695
                     /locus_tag="PSW2_8"
                     /label=PSW2_8