LOCUS       Exported                3877 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGFIB-1 DNA, complete sequence.
ACCESSION   LC121522
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Okamoto S, Niki H.
  TITLE     pGFIB-1 vector sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Okamoto S, Niki H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2016) Contact:Sho Okamoto National Institute of 
            Genetics, Microbial Genetics Laboratory, Genetics Strains Reseach 
            Center; 1111 Yata, Mishima, Shizuoka 411-8540, Japan URL 
            :http://www.nig.ac.jp/labs/MicroGen/index.html
REFERENCE   3  (bases 1 to 3877)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Cloning vector pGFIB-1"
                     /mol_type="other DNA"
                     /db_xref="taxon:1789745"
     CDS             complement(1..861)
                     /codon_start=1
                     /gene="amp marker"
                     /label=amp marker
                     /protein_id="BAU45358.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(1..861)
                     /gene="amp marker"
                     /label=amp marker
     promoter        complement(862..966)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1713..2226)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      1903..2209
                     /label=f1 origin
                     /note="f1 origin"
     primer_bind     2667..2683
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3104..3723
                     /label=pMB1 origin
                     /note="pMB1 origin"
     rep_origin      complement(3119..3707)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"