LOCUS       Exported                3130 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGEM-WIZ, complete sequence.
ACCESSION   DQ917671
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Bao S, Cagan R.
  TITLE     Fast cloning inverted repeats for RNA interference
  JOURNAL   RNA 12 (11), 2020-2024 (2006)
  PUBMED    17005926
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Bao S, Cagan R.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2006) Molecular Biology and Pharmacology, 
            Washington University, 660 South Euclid Ave, St. Louis, MO 63110, 
            USA
REFERENCE   3  (bases 1 to 3130)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Cloning vector pGEM-WIZ"
                     /mol_type="other DNA"
                     /note="constructed by removing Drosophila white intron 
                     2-containing XhoI/XbaI fragment of pWIZ and inserting it 
                     into pGEM-S1"
                     /db_xref="taxon:404006"
     misc_feature    10..243
                     /label=multiple cloning region
                     /note="multiple cloning region"
     regulatory      33..42
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     intron          77..150
                     /number=2
                     /note="white gene"
     regulatory      254..273
                     /regulatory_class="promoter"
                     /note="SP6 RNA polymerase promoter"
     promoter        complement(255..273)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(291..307)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    315..331
                     /label=lac operator
                     /note="lac operator"
     protein_bind    315..331
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(339..369)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    384..405
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(693..1281)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1452..2312)
                     /codon_start=1
                     /gene="beta-lactamase"
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="ABI82062.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(1452..2312)
                     /gene="beta-lactamase"
                     /label=beta-lactamase
     promoter        complement(2313..2417)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    2495..2950
                     /label=phage f1 region
                     /note="phage f1 region"
     rep_origin      complement(2495..2950)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     3091..3107
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      3114..3
                     /regulatory_class="promoter"
                     /note="T7 RNA polymerase promoter"
     promoter        3114..2
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"