LOCUS       Exported                4350 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGEM-URA3, complete sequence.
ACCESSION   AF173954
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Wilson RB, Davis D, Mitchell AP.
  TITLE     Rapid hypothesis testing with Candida albicans through gene 
            disruption with short homology regions
  JOURNAL   J. Bacteriol. 181 (6), 1868-1874 (1999)
  PUBMED    10074081
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Wilson RB, Davis D, Mitchell AP.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-1999) Microbiology, Columbia University, 701 W. 
            168th St., New York, NY 10032, USA
REFERENCE   3  (bases 1 to 4350)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Cloning vector pGEM-URA3"
                     /lab_host="Candida albicans SC5314"
                     /mol_type="genomic DNA"
                     /label=Candida albicans URA3 gene cloned into pGEM-T
                     /note="Candida albicans URA3 gene cloned into pGEM-T"
                     /db_xref="taxon:101832"
     promoter        complement(75..93)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(111..127)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    135..151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(159..189)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    204..225
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(513..1101)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1272..2132)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2133..2237)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2318..2773)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     2914..2930
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2937..2955
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2986..2995
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3409..4221
                     /codon_start=1
                     /gene="URA3"
                     /product="orotidine-5-phosphate decarboxylase"
                     /label=URA3
                     /protein_id="AAF00226.1"
                     /translation="MTVNTKTYSERAETHASPVAQRLFRLMELKKTNLCASIDVDTTKE
                     FLELIDKLGPYVCLIKTHIDIINDFSYESTIEPLLELSRKHQFMIFEDRKFADIGNTVK
                     KQYIGGVYKISSWADITNAHGVTGNGVVEGLKQGAKETTTNQEPRGLLMLAELSSVGSL
                     AYGEYSQKTVEIAKSDKEFVIGFIAQRDMGGQEEGFDWLIMTPGVGLDDKGDGLGQQYR
                     TVDEVVSTGTDIIIVGRGLFGKGRDPDIEGKRYRNAGWNAYLKKTGQL"
     gene            3409..4221
                     /gene="URA3"
                     /label=URA3