LOCUS       Exported                3357 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGCAP10 DNA, complete sequence.
ACCESSION   AB371573
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Oshikawa M, Sugai Y, Usami R, Ohtoko K, Toyama S, Kato S.
  TITLE     Fine Expression Profiling of Full-length Transcripts using a 
            Size-unbiased cDNA Library Prepared with the Vector-capping Method
  JOURNAL   DNA Res. 15 (3), 123-136 (2008)
  PUBMED    18487259
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Kato S.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2007) Contact:Seishi Kato National Rehabilitation 
            Center for Persons with Disabilities, Research Institute, Department
            of Rehabilitation Engineering; 4-1 Namiki, Tokorozawa, Saitama 
            359-8555, Japan
REFERENCE   3  (bases 1 to 3357)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Cloning vector pGCAP10"
                     /mol_type="other DNA"
                     /note="Cloning vector pGCAP10 used for the vector-capping 
                     method"
                     /db_xref="taxon:489442"
     promoter        18..347
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      198..333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          386..482
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     526..542
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        550..568
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    601..606
                     /label=KpnI site for dT tailing
                     /note="KpnI site for dT tailing"
     promoter        complement(622..640)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    642..776
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1074..1662)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1833..2693)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2694..2798)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2825..3280)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     3298..3314
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"