LOCUS       Exported                7942 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Agrobacterium binary gap repair vector pGAP-Hyg, complete sequence.
ACCESSION   EU933993
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Bitrian M, Roodbarkelari F, Horvath M, Koncz C.
  TITLE     BAC-recombineering for studying plant gene regulation: developmental
            control and cellular localization of SnRK1 kinase subunits
  JOURNAL   Plant J. 65 (5), 829-842 (2011)
  PUBMED    21235649
REFERENCE   2  (bases 1 to 7942)
  AUTHORS   Bitrian M, Koncz C.
  TITLE     Agrobacterium binary gap repair vectors for recombineering with 
            Arabidopsis BACs
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7942)
  AUTHORS   Bitrian M, Koncz C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2008) Plant Developmental Biology, Max-Planck 
            Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, 
            Cologne, NRW 50829, Germany
REFERENCE   4  (bases 1 to 7942)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7942
                     /organism="Agrobacterium binary gap repair vector
                     pGAP-Hyg"
                     /mol_type="other DNA"
                     /db_xref="taxon:556553"
     misc_feature    1..59
                     /note="polylinker: EcoRI, HindIII, HindIII, XbaI, BglII, 
                     PstI, SalI, XbaI, BamHI"
     misc_feature    64..383
                     /note="derived from Escherichia coli transposon Tn5 
                     sequence in GenBank Accession Number U00004"
     regulatory      384..674
                     /regulatory_class="promoter"
                     /note="Agrobacterium str. C58 Ti plasmid 
                     (gb|AE007871.2)T-DNA encoded nopaline synthase (NOS; 
                     D-nopaline dehydrogenase) gene promoter"
     promoter        467..646
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             691..1716
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=hygromycin B phosphotransferase
                     /note="hpt [aph(4)]; derived from an EcoRI site modified 
                     hygromycin B phosphotransferase [aph(4)] gene coding 
                     region"
                     /protein_id="ACH73256.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    1747..2000
                     /note="Agrobacterium str. C58 Ti plasmid (gb|AE007871.2) 
                     T-DNA encoded nopaline synthase (NOS; D-nopaline 
                     dehydrogenase) gene polyadenylation sequences"
     terminator      1747..1999
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2014..3012
                     /note="Agrobacterium str. C58 Ti plasmid (gb|AE007871.2) 
                     T-DNA right border region"
     misc_feature    2059..2084
                     /note="Agrobacterium str. C58 Ti plasmid T-DNA right 
                     border; 25bp repeat"
     misc_feature    2059..2083
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    3026..3944
                     /note="Birmingham IncP-alpha plasmid RK2 
                     (gb|L27758.1|BIACOMGEN; NC_001621) traJXIH/oriT region"
     oriT            3211..3320
                     /note="incP origin of transfer"
     misc_feature    3317..3318
                     /label=oriT nick site
                     /note="oriT nick site"
     rep_origin      3945..4828
                     /note="Birmingham IncP-alpha plasmid RK2 
                     (gb|L27758.1|BIACOMGEN; NC_001621) oriV replication origin;
                     TrfA binding repeats"
     rep_origin      4114..4825
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    4829..5124
                     /note="derived from Birmingham IncP-alpha plasmid RK2 
                     (gb|L27758.1|BIACOMGEN; NC_001621)"
     misc_feature    5125..5715
                     /note="Agrobacterium str. Ach5 Ti plasmid T-DNA left border
                     region KpnI-ClaI fragment"
     misc_feature    5412..5437
                     /note="Agrobacterium str. Ach5 Ti plasmid T-DNA left 
                     border; 25bp repeat"
     misc_feature    5412..5436
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    5715..7942
                     /label=derived from pBluescript SKII(-)
                     /note="derived from pBluescript SKII(-)"
     rep_origin      complement(5924..6512)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6683..7543)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMQTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7544..7648)
                     /gene="bla"
                     /label=AmpR promoter