LOCUS       Exported                4716 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Phagemid vector pGALD9DLFN, complete sequence.
ACCESSION   HQ528250
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Loset GA, Roos N, Bogen B, Sandlie I.
  TITLE     Expanding the versatility of phage display II: improved affinity 
            selection of folded domains on protein VII and IX of the filamentous
            phage
  JOURNAL   PLoS ONE 6 (2), E17433 (2011)
  PUBMED    21390283
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Loset GA, Roos N, Bogen B, Sandlie I.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2010) Department of Molecular Biosciences, 
            University of Oslo, PO Box 1041 Blindern, Oslo N-0316, Norway
REFERENCE   3  (bases 1 to 4716)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Phagemid vector pGALD9DLFN"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:913417"
     protein_bind    31..52
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        67..97
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..121
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             140..151
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             158..1033
                     /codon_start=1
                     /gene="scTCR-pIX"
                     /product="recombinant single chain T cell receptor pIX 
                     fusion protein"
                     /label=scTCR-pIX
                     /protein_id="ADQ12904.1"
                     /translation="MAQQKVQQSPESLIVPEGAMTSLNCTFSDSASQYFAWYRQQSGKA
                     PKALMSIFSNGEKEEGRFTIHLNKASLHFSLHIRDSQPSDSALYLCAVRGPNTGNYKYV
                     FGAGTRLKVIAKLSGSASAPKLEEGEFSEARVEAAVTQSPRNKVAVTGGKVTLSCNQTN
                     NHNNMYWYRQDTGHGLRLIHYSYGAGSTEKGDIPDGYKASRPSQENFSLILELATPSQT
                     SVYFCASGDAGQGHSDYTFGSGTRLLVIAAAGSKDIRMSVLVYSFASFVLGWCLRSGIT
                     YFTRLMETSS"
     gene            158..1033
                     /gene="scTCR-pIX"
                     /label=scTCR-pIX
     misc_feature    164..907
                     /gene="scTCR-pIX"
                     /label=Region: scTCR
                     /note="Region: scTCR"
     CDS             935..1033
                     /codon_start=1
                     /gene="IX"
                     /product="pIX"
                     /label=M13 gene IX
                     /note="minor coat protein"
                     /translation="MSVLVYSFASFVLGWCLRSGITYFTRLMETSS"
     misc_feature    935..1030
                     /gene="scTCR-pIX"
                     /label=Region: pIX
                     /note="Region: pIX"
     CDS             complement(1112..1951)
                     /codon_start=1
                     /gene="rFkpA"
                     /product="recombinant FkpA"
                     /label=rFkpA
                     /protein_id="ADQ12905.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATAADSKAAFKNDD
                     QKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTLQA
                     FEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEAPKD
                     SDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLVIPPE
                     LAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKKDYKDDDDKR"
     gene            complement(1112..1951)
                     /gene="rFkpA"
                     /label=rFkpA
     CDS             complement(1118..1141)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     rep_origin      2249..2704
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2730..2834
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2835..3695
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3866..4454
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"