LOCUS       Exported                3736 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Phagemid vector pGALD9, complete sequence.
ACCESSION   HQ528248
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Loset GA, Roos N, Bogen B, Sandlie I.
  TITLE     Expanding the versatility of phage display II: improved affinity 
            selection of folded domains on protein VII and IX of the filamentous
            phage
  JOURNAL   PLoS ONE 6 (2), E17433 (2011)
  PUBMED    21390283
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Loset GA, Roos N, Bogen B, Sandlie I.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2010) Department of Molecular Biosciences, 
            University of Oslo, PO Box 1041 Blindern, Oslo N-0316, Norway
REFERENCE   3  (bases 1 to 3736)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Phagemid vector pGALD9"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:913415"
     protein_bind    31..52
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        67..97
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..121
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             140..151
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             158..1108
                     /codon_start=1
                     /gene="pelBss/scFv-pIX fusion"
                     /product="recombinant single chain antibody-pIX fusion 
                     protein"
                     /label=pelBss/scFv-pIX fusion
                     /protein_id="ADQ12902.1"
                     /translation="MKYLLPTAAAGLLLLAAQPAMAQVQLVQSGGEVKKPGASVKVSCK
                     ASGYTFTNYGITWVRQAPGQGLEWMGWISAYNGYKSYAQNFQGRVTMTTDTFTSTAYME
                     LRTLRSDDTAVYYCVRLVPKRTATLHYYIDVWGKGTTVTVSSGSASAPKLEEGEFSEAR
                     VQSVLTQPPSVSAAPGQKVTISCSGSSSNIGNNYVSWYVQLPGTAPKLLIYDNNKRFSG
                     VPDRFSGSKSGTSATLGITGLQTGDEADYYCGAWDGSLREAVFGGGTKVTVLGAAAGSK
                     DIRMSVLVYSFASFVLGWCLRSGITYFTRLMETSS"
     gene            158..1108
                     /gene="pelBss/scFv-pIX fusion"
                     /label=pelBss/scFv-pIX fusion
     sig_peptide     158..223
                     /gene="pelBss/scFv-pIX fusion"
                     /note="from pelBss protein"
     CDS             158..223
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     misc_feature    224..982
                     /gene="pelBss/scFv-pIX fusion"
                     /label=Region: scFv
                     /note="Region: scFv"
     CDS             1010..1108
                     /codon_start=1
                     /gene="IX"
                     /product="pIX"
                     /label=M13 gene IX
                     /note="minor coat protein"
                     /translation="MSVLVYSFASFVLGWCLRSGITYFTRLMETSS"
     misc_feature    1010..1105
                     /gene="pelBss/scFv-pIX fusion"
                     /label=Region: pIX
                     /note="Region: pIX"
     terminator      1129..1172
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      1269..1724
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1750..1854
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1855..2715
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2886..3474
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"