LOCUS       Exported                4887 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pGal4-VP16, complete sequence.
ACCESSION   LT727167
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4887)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Mammalian expression vector pGal4-VP16"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 4708."
                     /db_xref="taxon:1945044"
     misc_feature    1..825
                     /label=SV40 VP1 with internal deletion
                     /note="SV40 VP1 with internal deletion"
     polyA_signal    766..900
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(826..900)
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     regulatory      826..900
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     CDS             complement(1059..1295)
                     /codon_start=1
                     /note="unnamed protein product; VPad"
                     /protein_id="SJL87647.1"
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             complement(1062..1295)
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     misc_feature    complement(1296..1316)
                     /label=linker
                     /note="linker"
     CDS             complement(1317..1754)
                     /codon_start=1
                     /note="unnamed protein product; GALbd"
                     /protein_id="SJL87648.1"
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     regulatory      complement(1898..2079)
                     /regulatory_class="promoter"
                     /note="RSV-LTR"
     promoter        complement(1899..2160)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     regulatory      2476..2757
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     promoter        2485..2814
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2665..2800
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      2714..2789
                     /label=SV40 ori
                     /note="SV40 ori"
     rep_origin      complement(2876..3278)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(3060..3648)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3819..4679)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin"
                     /protein_id="SJL87649.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4680..4784)
                     /gene="bla"
                     /label=AmpR promoter