LOCUS       Exported                6101 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pGal4-p65f286-550, complete sequence.
ACCESSION   LT727168
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6101)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6101)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Mammalian expression vector pGal4-p65f286-550"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 4709."
                     /db_xref="taxon:1945036"
     misc_feature    1..825
                     /label=SV40 VP1 with internal deletion
                     /note="SV40 VP1 with internal deletion"
     polyA_signal    766..900
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(826..900)
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     regulatory      826..900
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     misc_feature    complement(934..1723)
                     /label=3 UTR of hNF-kappaB p65 subunit
                     /note="3 UTR of hNF-kappaB p65 subunit"
     CDS             complement(1724..2527)
                     /codon_start=1
                     /note="unnamed protein product; hNF-kappaB p65 subunit 
                     (285-551)"
                     /protein_id="SJL87650.1"
                     /translation="EFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPR
                     RIAVPSRSSASVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLP
                     QAPAPAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDD
                     EDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTG
                     AQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS"
     CDS             complement(1727..2098)
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (OShea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     CDS             complement(2531..2968)
                     /codon_start=1
                     /note="unnamed protein product; GALbd"
                     /protein_id="SJL87651.1"
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     regulatory      complement(3112..3293)
                     /regulatory_class="promoter"
                     /note="RSV-LTR"
     promoter        complement(3113..3374)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     regulatory      3690..3971
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     promoter        3699..4028
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3879..4014
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      3928..4003
                     /label=SV40 ori
                     /note="SV40 ori"
     rep_origin      complement(4090..4492)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(4274..4862)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5033..5893)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin"
                     /protein_id="SJL87652.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5894..5998)
                     /gene="bla"
                     /label=AmpR promoter