LOCUS       Exported                5334 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGABI1, complete sequence.
ACCESSION   AY529716
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Li Y, Rosso MG, Strizhov N, Viehoever P, Weisshaar B.
  TITLE     GABI-Kat SimpleSearch: a flanking sequence tag (FST) database for 
            the identification of T-DNA insertion mutants in Arabidopsis 
            thaliana
  JOURNAL   Bioinformatics 19 (11), 1441-1442 (2003)
  PUBMED    12874060
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Rosso MG, Li Y, Strizhov N, Reiss B, Dekker K, Weisshaar B.
  TITLE     An Arabidopsis thaliana T-DNA mutagenized population (GABI-Kat) for 
            flanking sequence tag-based reverse genetics
  JOURNAL   Plant Mol. Biol. 53 (1-2), 247-259 (2003)
  PUBMED    14756321
REFERENCE   3  (bases 1 to 5334)
  AUTHORS   Rosso MG, Li Y, Strizhov N, Weisshaar B.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2004) Dept. Salamini, Max-Planck-Institute for 
            Plant Breeding Research, Carl-von-Linne-Weg 10, Koeln D-50829, 
            Germany
REFERENCE   4  (bases 1 to 5334)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Cloning vector pGABI1"
                     /mol_type="other DNA"
                     /db_xref="taxon:264698"
     rep_origin      31..740
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      35..743
                     /label=oriV
                     /note="oriV"
     promoter        813..904
                     /gene="bla"
                     /label=AmpR promoter
     CDS             905..1765
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="bla; ampicillin resistance"
                     /protein_id="AAS48632.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1834..2526
                     /label=OriColE1
                     /note="OriColE1"
     rep_origin      1936..2524
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2832..2856
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    2832..2855
                     /label=T-DNA left border
                     /note="T-DNA left border"
     rep_origin      3546..4025
                     /label=1#-2# eucaryotic promoter
                     /note="1#-2# eucaryotic promoter"
     promoter        complement(3546..3926)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     regulatory      4073..4243
                     /regulatory_class="other"
                     /note="pea rubisco targeting signal"
     CDS             4244..5083
                     /codon_start=1
                     /product="dihydropteroate synthase"
                     /label=dihydropteroate synthase
                     /note="sul1"
                     /protein_id="AAS48633.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDVG
                     PAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLNDIQ
                     GFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSALRRS
                     GVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGATVGLP
                     VKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITISETLAKFRSRDARDRGLDHA"
     misc_feature    5311..1
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    5311..5334
                     /label=T-DNA right border
                     /note="T-DNA right border"