LOCUS       Exported               13476 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pGA1611, complete sequence.
ACCESSION   AY373338
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13476)
  AUTHORS   Kim S-R., Lee S, Kang H-G., Jeon J-S., Kim K-M., An G.
  TITLE     A complete sequence of the pGA1611 binary vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13476)
  AUTHORS   Kim S-R., Lee S, Kang H-G., Jeon J-S., Kim K-M., An G.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2003) Life Science, POSTECH, Nam-Gu Hyoja San31, 
            Pohang, Kyungbuk 790-784, Republic of Korea
REFERENCE   3  (bases 1 to 13476)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13476
                     /organism="Binary vector pGA1611"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:245199"
     misc_feature    1..25
                     /label=right T-DNA border
                     /note="right T-DNA border"
     promoter        149..328
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        181..364
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        420..2412
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     regulatory      420..2406
                     /regulatory_class="promoter"
                     /note="maize ubiquitin promoter"
     misc_feature    2407..2451
                     /label=polylinker
                     /note="polylinker"
     3UTR           2463..2715
                     /label=derived from nos
                     /note="derived from nos"
     terminator      2463..2715
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     polyA_signal    3252..3299
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     regulatory      3366..4108
                     /regulatory_class="promoter"
                     /note="35S promoter of CaMV"
     promoter        3767..4111
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             4151..5176
                     /codon_start=1
                     /gene="hph"
                     /product="hygromycin phosphotransferase"
                     /label=hph
                     /protein_id="AAQ76848.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     gene            4151..5176
                     /gene="hph"
                     /label=hph
     regulatory      5199..5410
                     /regulatory_class="terminator"
                     /note="derived from gene 7"
     terminator      5210..5410
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    5553..5577
                     /label=left T-DNA border
                     /note="left T-DNA border"
     oriT            6132..6241
                     /label=incP origin of transfer
                     /note="incP origin of transfer"
     CDS             6274..6645
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /protein_id="AAQ76849.1"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     gene            6274..6645
                     /gene="traJ"
                     /label=traJ
     CDS             6917..8065
                     /codon_start=1
                     /gene="trfA"
                     /product="trans-acting replication"
                     /label=trfA
                     /protein_id="AAQ76850.1"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     gene            6917..8065
                     /gene="trfA"
                     /label=trfA
     CDS             complement(9338..10537)
                     /codon_start=1
                     /gene="tetA"
                     /product="TetA"
                     /function="tetracycline resistance"
                     /label=tetA
                     /protein_id="AAQ76851.1"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     gene            complement(9338..10537)
                     /gene="tetA"
                     /label=tetA
     CDS             10643..11293
                     /codon_start=1
                     /gene="tetR"
                     /product="TetR"
                     /function="tetracycline resistance regulation"
                     /label=tetR
                     /protein_id="AAQ76852.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            10643..11293
                     /gene="tetR"
                     /label=tetR
     rep_origin      12288..12996
                     /note="OriV; incP origin of replication"
     rep_origin      12358..12989
                     /label=oriV
                     /note="incP origin of replication"