LOCUS       Exported               11166 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway adapted binary vector pFYTAG, complete sequence.
ACCESSION   DQ370421
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11166)
  AUTHORS   Zhang C, Galbraith DW.
  TITLE     A Gateway adapted Agrobacterium binary vector series for plant 
            biological studies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11166)
  AUTHORS   Zhang C, Galbraith DW.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2006) Department of Plant Sciences, University of 
            Arizona, 303 Forbes Building, Tucson, AZ 85721-0036, USA
REFERENCE   3  (bases 1 to 11166)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11166
                     /organism="Gateway adapted binary vector pFYTAG"
                     /mol_type="other DNA"
                     /db_xref="taxon:370580"
     misc_feature    1..6517
                     /label=derived from plasmid pFYFPBT
                     /note="derived from plasmid pFYFPBT"
     CDS             425..1219
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=aminoglycoside phosphotransferase
                     /note="aadA (kanamycin resistance) amplified from pIG121Hm"
                     /protein_id="ABD16407.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      1306..1894
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      complement(1509..1790)
                     /direction=LEFT
                     /label=pBR325 replication origin
                     /note="pBR325 replication origin"
     misc_feature    2080..2220
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      2564..2758
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2824..3891)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      4193..5193
                     /note="pVS1-REP; replication origin from pVS1"
     CDS             complement(4325..4954)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    6254..6278
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     repeat_region   6255..6278
                     /label=right border repeat from C58 T-DNA
                     /note="right border repeat from C58 T-DNA"
     primer_bind     6481..6497
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6518..8231
                     /label=Invitrogen attR cassette (reading frame C.1)
                     /note="Invitrogen attR cassette (reading frame C.1)"
     protein_bind    6523..6647
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        6672..6702
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             6756..7436
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             7756..8061
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(8102..8226)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    8232..11166
                     /label=derived from plasmid pFYFPBT
                     /note="derived from plasmid pFYFPBT"
     5UTR           8232..8366
                     /note="translation leader sequence; 5UTR from tobacco etch
                     virus"
     CDS             8368..9549
                     /codon_start=1
                     /product="HTA6-EYFP"
                     /label=HTA6-EYFP
                     /note="translational fusion between HTA6 (Arabidopsis 
                     histone 2A, At5g59870) and enhanced yellow fluorescent 
                     protein (EYFP)"
                     /protein_id="ABD16408.1"
                     /translation="MESTGKVKKAFGGRKPPGAPKTKSVSKSMKAGLQFPVGRITRFLK
                     KGRYAQRLGGGAPVYMAAVLEYLAAEVLELAGNAARDNKKSRIIPRHLLLAIRNDEELG
                     KLLSGVTIAHGGVLPNINSVLLPKKSATKPAEEKATKSPVKSPKKAAMVSKGEELFTGV
                     VPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLQC
                     FARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGID
                     FKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPI
                     GDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKRSI"
     CDS             8821..9537
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    9551..9620
                     /note="computer generated random sequence as a tag of the 
                     HTA6-EYFP fusion transcript"
     regulatory      9625..9835
                     /regulatory_class="polyA_signal_sequence"
                     /note="cauliflower mosaic virus 35S poly(A) signal"
     regulatory      9857..10237
                     /regulatory_class="promoter"
                     /note="mannopine synthase promoter"
     promoter        9857..10237
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             10243..10794
                     /codon_start=1
                     /product="phosphinothricin acetyltransferase"
                     /label=phosphinothricin acetyltransferase
                     /note="BAR; conferring tolerance to the herbicide 
                     phosphinothricin (PPT)"
                     /protein_id="ABD16409.1"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      10804..11056
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     regulatory      10805..11056
                     /regulatory_class="polyA_signal_sequence"
                     /note="mannopine synthase poly(A) signal"
     repeat_region   11140..11166
                     /label=left border repeat from C58 T-DNA
                     /note="left border repeat from C58 T-DNA"
     misc_feature    11142..11166
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"