LOCUS       Exported                3585 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFYS018, complete sequence.
ACCESSION   KX904358
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Scott FY, Ruder WC.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2016) Biological Systems Engineering, Virginia 
            Polytechnic Institute and State University, 1230 Washington Street 
            SW, Blacksburg, VA 24061, USA
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Cloning vector pFYS018"
                     /mol_type="other DNA"
                     /country="USA"
                     /db_xref="taxon:2033674"
     regulatory      151..435
                     /regulatory_class="promoter"
                     /note="araBAD"
     promoter        151..435
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     regulatory      442..452
                     /regulatory_class="ribosome_binding_site"
     CDS             459..1448
                     /codon_start=1
                     /product="lpp-ompA-SNAP-tag(R)"
                     /label=lpp-ompA-SNAP-tag
                     /protein_id="ASY93072.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQEFNPYVGFEMGYDWLG
                     RMPYKGSVENGAYKAQGVQLTAKLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTG
                     VSPVFAGGVEYAITPEIATRLEYQWTNNIGDAHTIGTRPDNELMDKDCEMKRTTLDSPL
                     GKLELSGCEQGLHEIKLLGKGTSAADAVEVPAPAAVLGGPEPLMQATAWLNAYFHQPEA
                     IEEFPVPALHHPVFQQESFTRQVLWKLLKVVKFGEVISYQQLAALAGNPAATAAVKTAL
                     SGNPVPILIPCHRVVSSSGAVGGYEGGLAVKEWLLAHEGHRLGKPGLG"
     CDS             900..1445
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="optimized for bacterial expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYQQLAALAGNPAATAAVKTALSGNPVPILIPCHRVVSSSGAVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     regulatory      1545..1631
                     /regulatory_class="terminator"
                     /note="rrnB T1"
     terminator      1545..1631
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      1795..2383
     regulatory      2471..2565
                     /regulatory_class="terminator"
                     /note="lambda t0"
     terminator      2471..2565
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     gene            complement(2595..3560)
                     /gene="ampR"
                     /label=ampR
     CDS             complement(2595..3455)
                     /codon_start=1
                     /gene="ampR"
                     /product="AmpR"
                     /label=ampR
                     /protein_id="ASY93073.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     regulatory      complement(3456..3560)
                     /regulatory_class="promoter"
                     /gene="ampR"
     promoter        complement(3456..3560)
                     /gene="bla"
                     /label=AmpR promoter