LOCUS       Exported                6807 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFY20, complete sequence.
ACCESSION   EU665638
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Wahls WP, Davidson MK.
  TITLE     Low-copy episomal vector pFY20 and high-saturation coverage genomic 
            libraries for the fission yeast Schizosaccharomyces pombe
  JOURNAL   Yeast 25 (9), 643-650 (2008)
  PUBMED    18613214
REFERENCE   2  (bases 1 to 6807)
  AUTHORS   Wahls WP, Davidson MK.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2008) Biochemistry and Molecular Biology, 
            University of Arkansas for Medical Sciences, 4301 West Markham 
            Street (slot 516), Little Rock, AR 72205-7199, USA
REFERENCE   3  (bases 1 to 6807)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6807
                     /organism="Cloning vector pFY20"
                     /mol_type="other DNA"
                     /note="replicates in fission yeast Schizosaccharomyces 
                     pombe"
                     /db_xref="taxon:526520"
     misc_feature    237..1551
                     /note="stb; plasmid stability element derived from 
                     Schizosaccharomyces pombe"
     rep_origin      1552..2479
                     /note="ars1; derived from Schizosaccharomyces pombe"
     rep_origin      1900..2681
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     CDS             3332..4126
                     /codon_start=1
                     /product="Ura4"
                     /EC_number="4.1.1.23"
                     /label=Ura4
                     /note="orotidine 5-phosphate decarboxylase"
                     /protein_id="ACD14087.1"
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     primer_bind     complement(4586..4602)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4610..4626
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4634..4664)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4679..4700
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      4931..5041
                     /label=ori derived from pBR322 of Escherichia coli
                     /note="ori derived from pBR322 of Escherichia coli"
     rep_origin      complement(4988..5576)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5747..6607)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="ACD14088.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6608..6712)
                     /gene="bla"
                     /label=AmpR promoter