LOCUS       Exported                3230 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter gene-fusion vector pFU95, complete sequence.
ACCESSION   JF796092
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Uliczka F, Pisano F, Kochut A, Opitz W, Herbst K, Stolz T, Dersch P.
  TITLE     Monitoring of Gene Expression in Bacteria during Infections Using an
            Adaptable Set of Bioluminescent, Fluorescent and Colorigenic Fusion 
            Vectors
  JOURNAL   PLoS ONE 6 (6), E20425 (2011)
  PUBMED    21673990
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Uliczka F.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2011) Department of Molecular Infection Biology, 
            Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 
            Braunschweig, Niedersachsen 38124, Germany
REFERENCE   3  (bases 1 to 3230)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Reporter gene-fusion vector pFU95"
                     /mol_type="other DNA"
                     /db_xref="taxon:1047788"
     regulatory      12..357
                     /regulatory_class="promoter"
                     /note="derived from Escherichia coli gapA"
     gene            365..1095
                     /gene="gfpmut3.1"
                     /label=gfpmut3.1
     regulatory      365..370
                     /regulatory_class="ribosome_binding_site"
                     /gene="gfpmut3.1"
     CDS             379..1095
                     /codon_start=1
                     /gene="gfpmut3.1"
                     /product="Gfpmut3.1"
                     /label=gfpmut3.1
                     /protein_id="AEI59090.1"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1115..1201
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      1357..2027
                     /label=colE1
                     /note="colE1"
     rep_origin      complement(1365..1953)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      2041..2135
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2165..3025)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampicillin-resistance protein"
                     /protein_id="AEI59091.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2165..3025)
                     /gene="bla"
                     /label=bla
     promoter        complement(3026..3130)
                     /gene="bla"
                     /label=AmpR promoter