LOCUS       Exported                3197 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter gene-fusion vector pFU168, complete sequence.
ACCESSION   JF796094
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Uliczka F, Pisano F, Kochut A, Opitz W, Herbst K, Stolz T, Dersch P.
  TITLE     Monitoring of Gene Expression in Bacteria during Infections Using an
            Adaptable Set of Bioluminescent, Fluorescent and Colorigenic Fusion 
            Vectors
  JOURNAL   PLoS ONE 6 (6), E20425 (2011)
  PUBMED    21673990
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Uliczka F.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2011) Department of Molecular Infection Biology, 
            Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 
            Braunschweig, Niedersachsen 38124, Germany
REFERENCE   3  (bases 1 to 3197)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Reporter gene-fusion vector pFU168"
                     /mol_type="other DNA"
                     /db_xref="taxon:1047770"
     gene            37..767
                     /gene="gfpmut3.1"
                     /label=gfpmut3.1
     regulatory      37..42
                     /regulatory_class="ribosome_binding_site"
                     /gene="gfpmut3.1"
     CDS             51..767
                     /codon_start=1
                     /gene="gfpmut3.1"
                     /product="Gfpmut3.1"
                     /label=gfpmut3.1
                     /protein_id="AEI59098.1"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      787..873
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      1029..1699
                     /label=colE1
                     /note="colE1"
     rep_origin      complement(1037..1625)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      1713..1807
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1850..3040)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance protein"
                     /label=tetR
                     /protein_id="AEI59099.1"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     gene            complement(1850..3040)
                     /gene="tetR"
                     /label=tetR
     promoter        complement(3088..3116)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"