LOCUS       Exported                9158 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFtsZ-SYFP2, complete sequence.
ACCESSION   MH050937
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9158)
  AUTHORS   Zhang JY, Lin GM, Xing WY, Zhang CC.
  TITLE     Diversity of Growth Patterns Probed in Live Cyanobacterial Cells 
            Using a Fluorescent Analog of a Peptidoglycan Precursor
  JOURNAL   Front Microbiol 9, 791 (2018)
  PUBMED    29740419
REFERENCE   2  (bases 1 to 9158)
  AUTHORS   Zhang J-Y., Lin G-M., Xing W-Y., Zhang C-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2018) Institute of Hydrobiology, Donghu Nanlu, 
            Wuhan, Hubei 430072, 086
REFERENCE   3  (bases 1 to 9158)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9158
                     /organism="Cloning vector pFtsZ-SYFP2"
                     /mol_type="other DNA"
                     /db_xref="taxon:2201265"
     CDS             complement(473..1894)
                     /codon_start=1
                     /gene="sacB"
                     /product="levansucrase"
                     /label=sacB
                     /note="derived from Bacillus subtilis"
                     /protein_id="AWK77094.1"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLEVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     gene            complement(473..1894)
                     /gene="sacB"
                     /label=sacB
     promoter        complement(1895..2340)
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     promoter        2488..2590
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2591..3250
                     /codon_start=1
                     /gene="cm"
                     /product="chloramphenicol resistance protein"
                     /label=cm
                     /protein_id="AWK77095.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            2591..3250
                     /gene="cm"
                     /label=cm
     misc_feature    3347..4471
                     /note="region for homologous recombination derived from 
                     Anabaena ftsZ"
     misc_feature    4487..4513
                     /label=domain linker
                     /note="domain linker"
     misc_feature    4514..5227
                     /label=super yellow fluorescent protein 2
                     /note="super yellow fluorescent protein 2"
     misc_feature    5228..5257
                     /label=domain linker
                     /note="domain linker"
     misc_feature    5258..5347
                     /label=TwinStrep affinity purification tag
                     /note="TwinStrep affinity purification tag"
     CDS             5264..5287
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5324..5347
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     terminator      5358..5405
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     regulatory      5358..5404
                     /regulatory_class="terminator"
     CDS             5646..6437
                     /codon_start=1
                     /gene="sp"
                     /product="spectinomycin and streptomycin resistance 
                     protein"
                     /label=sp
                     /protein_id="AWK77096.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            5646..6437
                     /gene="sp"
                     /label=sp
     regulatory      6495..6513
                     /regulatory_class="terminator"
     misc_feature    6595..7619
                     /note="region for homologous recombination derived from 
                     Anabaena ftsZ"
     rep_origin      8103..8691
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    8877..9017
                     /label=bom
                     /note="basis of mobility region from pBR322"