LOCUS       Exported                8928 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Lentiviral transfer vector pFTMGW, complete sequence.
ACCESSION   EF177827
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8928)
  AUTHORS   Geller SF, Ge PS, Visel M, Greenberg KP, Flannery JG.
  TITLE     Functional promoter testing using a modified lentiviral transfer 
            vector
  JOURNAL   Mol. Vis. 13, 730-739 (2007)
  PUBMED    17563724
REFERENCE   2  (bases 1 to 8928)
  AUTHORS   Geller SF, Ge PS.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2006) Helen Wills Neuroscience Institute, 
            University of California, Berkeley, 114 Barker Hall, Berkeley, CA 
            94720-3190, USA
REFERENCE   3  (bases 1 to 8928)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8928
                     /organism="Lentiviral transfer vector pFTMGW"
                     /mol_type="other DNA"
                     /note="a lentiviral transfer vector plasmid, modified from 
                     pFUGW"
                     /db_xref="taxon:436015"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     repeat_region   828..1016
                     /note="5 LTR (R and U5 portions; U3 was replaced by the 
                     CMV promoter)"
     LTR             835..1015
                     /label=5 LTR (truncated)
                     /note="truncated 5 long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1067..1204
                     /label=Psi
                     /note="Psi"
     misc_feature    1124..1127
                     /note="SD; Splice Donor"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2440..2557
                     /note="CPPT; central polypurine tract"
     misc_feature    2608..2761
                     /note="TB element; Transcription Blocker"
     polyA_signal    2608..2656
                     /note="synthetic polyadenylation signal"
     misc_feature    2670..2761
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     misc_feature    2767..2835
                     /note="MCS; Multiple Cloning Site"
     regulatory      2854..2863
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2860..3579
                     /codon_start=1
                     /gene="eGFP"
                     /product="enhanced green fluorescent protein"
                     /label=eGFP
                     /protein_id="ABP01834.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     gene            2860..3579
                     /gene="eGFP"
                     /label=eGFP
     misc_feature    3618..4211
                     /note="WPRE; Woodchuck post-transcriptional regulatory 
                     element"
     misc_feature    3620..4208
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(4091..4102)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4211..4227)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   4413..4914
                     /label=3 LTR from lentivirus
                     /note="3 LTR from lentivirus"
     LTR             4733..4913
                     /label=5 LTR (truncated)
                     /note="truncated 5 long terminal repeat (LTR) from HIV-1"
     polyA_signal    4945..5169
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5215..5643
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5657..5986
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5837..5972
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6034..6081
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6100..6474
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6604..6725
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6774..6790)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6798..6814
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6822..6852)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6867..6888
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7176..7764)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      7363..7588
                     /label=ColE1
                     /note="ColE1"
     CDS             complement(7935..8795)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8796..8900)
                     /gene="bla"
                     /label=AmpR promoter