LOCUS       Exported                8956 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Lentiviral transfer vector pFTM3GW, complete sequence.
ACCESSION   FJ797421
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8956)
  AUTHORS   Geller SF, Ge PS, Visel M, Flannery JG.
  TITLE     In vitro analysis of promoter activity in Muller cells
  JOURNAL   Mol. Vis. 14, 691-705 (2008)
  PUBMED    18437242
REFERENCE   2  (bases 1 to 8956)
  AUTHORS   Geller SF, Ge PS, Visel M, Flannery JG.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2009) Helen Wills Neuroscience Institute, 
            University of California, Berkeley, 114 Barker Hall, Berkeley, CA 
            94720, USA
REFERENCE   3  (bases 1 to 8956)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8956
                     /organism="Lentiviral transfer vector pFTM3GW"
                     /mol_type="other DNA"
                     /db_xref="taxon:627481"
     regulatory      260..850
                     /regulatory_class="promoter"
                     /note="CMV IE promoter"
     enhancer        320..623
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        625..823
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     repeat_region   834..1022
                     /label=5 LTR - Lentiviral long terminal repeat
                     /note="5 LTR - Lentiviral long terminal repeat"
     LTR             841..1021
                     /label=5 LTR (truncated)
                     /note="truncated 5 long terminal repeat (LTR) from HIV-1"
     misc_feature    1074..1199
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1079..1216
                     /label=PSI
                     /note="PSI"
     misc_feature    1136..1139
                     /label=SD - splice donor
                     /note="SD - splice donor"
     misc_feature    1692..1925
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2452..2569
                     /label=CPPT - central poly-purine tract
                     /note="CPPT - central poly-purine tract"
     regulatory      2620..2773
                     /regulatory_class="attenuator"
                     /note="TB - transcription blocker"
     polyA_signal    2620..2668
                     /note="synthetic polyadenylation signal"
     misc_feature    2682..2773
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     misc_feature    2789..2863
                     /label=MCS - multiple cloning site
                     /note="MCS - multiple cloning site"
     regulatory      2882..2891
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2888..3607
                     /codon_start=1
                     /gene="eGFP"
                     /product="enhanced green fluorescent protein"
                     /label=eGFP
                     /note="fluorescent reporter protein"
                     /protein_id="ACN91233.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     gene            2888..3607
                     /gene="eGFP"
                     /label=eGFP
     regulatory      3646..4239
                     /regulatory_class="enhancer"
                     /note="WPRE - Woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     misc_feature    3648..4236
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(4119..4130)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4239..4255)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   4441..4942
                     /label=3 LTR - Lentiviral long terminal repeat
                     /note="3 LTR - Lentiviral long terminal repeat"
     LTR             4761..4941
                     /label=5 LTR (truncated)
                     /note="truncated 5 long terminal repeat (LTR) from HIV-1"
     polyA_signal    4973..5197
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5243..5671
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5685..6014
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5865..6000
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6062..6109
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6128..6502
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6632..6753
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6802..6818)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6826..6842
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6850..6880)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6895..6916
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7204..7792)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      7391..7616
                     /label=ColE1 - bacterial origin of replication
                     /note="ColE1 - bacterial origin of replication"
     misc_feature    7866..8866
                     /label=AmpR - ampicillin resistance
                     /note="AmpR - ampicillin resistance"
     CDS             complement(7963..8823)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8824..8928)
                     /gene="bla"
                     /label=AmpR promoter