LOCUS       Exported                4205 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFS186-PCa2-LuxS, complete sequence.
ACCESSION   MF688632
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Sedlmayer F, Hell D, Mueller M, Auslaender D, Fussenegger M.
  TITLE     Designer cells programming quorum-sensing interference with human 
            pathogens
  JOURNAL   Nat Commun (2018) In press
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Sedlmayer F, Hell D, Mueller M, Auslaender D, Fussenegger M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2017) Department of Biosystems Science and 
            Engineering (D-BSSE), ETH Zurich, Mattenstrasse 26, Baseel 4058, 
            Switzerland
REFERENCE   3  (bases 1 to 4205)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Cloning vector pFS186-PCa2-LuxS"
                     /mol_type="other DNA"
                     /db_xref="taxon:2138156"
     CDS             9..524
                     /codon_start=1
                     /product="S-ribosylhomocysteine lyase"
                     /label=S-ribosylhomocysteine lyase
                     /note="LuxS"
                     /protein_id="AVR59179.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPNK
                     EVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVADAW
                     KAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELALPK
                     EKLQELHI"
     polyA_signal    563..684
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      787..1032
                     /regulatory_class="polyA_signal_sequence"
                     /note="simian virus 40 polyA"
     rep_origin      complement(1349..1937)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2108..2968)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2969..3073)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3100..3555
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     polyA_signal    3686..3734
                     /note="synthetic polyadenylation signal"
     misc_feature    3748..3839
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     regulatory      3884..4204
                     /regulatory_class="promoter"
                     /note="3xCRE-3xSRE-3xNFAT operator sequence fused to a 
                     synthetic minimal TATA-box minimal mammalian promoter Pmin;
                     PCa2"
     protein_bind    4071..4100
                     /label=NFAT binding site
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2 
                     (IL-2) gene (Mattila et al., 1990)"
     protein_bind    4101..4130
                     /label=NFAT binding site
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2 
                     (IL-2) gene (Mattila et al., 1990)"
     protein_bind    4131..4160
                     /label=NFAT binding site
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2 
                     (IL-2) gene (Mattila et al., 1990)"
     promoter        4173..4204
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"